Genotypic analysis of ticks species infesting cattle in Al-Diwaniyah abattoir

Mansoor J Ali,Saba F Khlaif,Wisam R Atiyah,Monyer A Al-Fatlawi

doi:10.33899/ijvs.2020.127772.1525

Abstract

Different vectors are considered critical for disease transmission between animals; however, ticks play a significant role in the dissemination of various infectious illnesses of animals and human importance. The current work was carried out to categorize ticks genetically of those isolated from cattle that entered Al-Diwaniyah abattoir. In the present study, 50 tick samples were collected and subjected to microscopic examination and genetic-based methods of polymerase chain reaction and partial gene sequencing, both utilized the mitochondrial cytochrome c oxidase subunit I (COX1) gene as a genotyping element. The findings of the microscopic examination showed that the ticks were from Hyalomma spp. Further analysis, the polymerase chain reaction revealed the genus of Hyalomma of the ticks, but when the PGS was performed, one sample of H. detritum, three samples of H. excavatum, and two samples of H. marginatum were identified. When the phylogenetic analyses were conducted, H. detritum showed close genetic similarity to an isolate from Spain EU827695.1. H. excavatum revealed similarity with isolates from India MK863382.1 and Turkey MT230050.1. In contrast, H. marginatum displayed close identity to an isolate from Iran (MG557555.1). In conclusion, these findings may indicate evolutionary links of the locally identified isolates to different world isolates, probably due to the trade-moving of animals.

Highlights

Ticks are the most diversified arthropod vectors for human and livestock animals
The polymerase chain reaction revealed the genus of Hyalomma of the ticks, but when the PGS was performed, one sample of H. detritum, three samples of H. excavatum, and two samples of H. marginatum were identified
Microscopic examination The findings of the ME showed that the ticks were from Hyalomma spp

Summary

Introduction

Ticks are the most diversified arthropod vectors for human and livestock animals. Among such, ticks contain the most numerous collection of infectious agents. Human tick-borne pathogens, similar to the tick-borne associated diseases of farm animal species, are zoonotic diseases of livestock origin, in the epidemiology and ecology of tickborne disease, complex interactions between the biotic and abiotic. A complex ecosystem of biotic and abiotic variables, including changes in animal and human demographics and behavior, influences both tick population densities and distributions. Aside from their immediate impact on ticks, a large number of tick-related micro-organisms and tick diseases, along with additional danger to public health of tick-like paralysis and other toxicity is affected by biotic and abiotic elements [7,8,9,10]. As ticks and transmitted diseases do not acknowledge foreign borders, every tick and tick-borne infection network should become part of any comprehensive foreign disease tracking program offering accurate, evidence-based coordination for health agencies, healthcare professionals, other decisionmakers, and stakeholders as well as the public [11,12]

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Results

Conclusion