Abstract
ObjectivesThe prevalence of drug-resistant TB in Shaanxi Province is higher than other areas. This study was aimed to investigate the genetic diversity and epidemiology of Mycobacterium tuberculosis clinical strains in Shaanxi Province, China.MethodsFrom January to December 2016, a total of 298 Mycobacterium tuberculosis clinical isolates from smear-positive pulmonary tuberculosis patients were genotyped by Mcspoligotyping and 15-locus VNTR.ResultsWe found that the Beijing family strains was the most prominent family(81.54%, 243/298). Other family strains included T family(9.06%, 27/298), U family(0.67%, 2/298), LAM9 family(0.34%, 1/298) and Manu family(0.34%, 1/298). The rates of multidrug-resistant (MDR) M.Tuberculosis, age, type of case and education between Beijing and non-Beijing family strains were not statistically different, while the distribution in the three different regions among these was statistically significant.VNTR results showed that strains were classified into 280 genotypes, and 33 (11.07%) strains could be grouped into 14 clusters. 11 of the 15-VNTR loci were highly or moderately discriminative according to the Hunter-Gaston discriminatory index.ConclusionsWe concluded that the Beijing family genotype was the most prevalent genotype and 15-locus VNTR typing might be suitable for genotyping of M. tuberculosis in Shaanxi Province. There was less association between Beijing family genotypes and drug resistance in our study area.
Highlights
Tuberculosis(TB) is a chronic respiratory infectious diseases caused by Mycobacterium tuberculosis(M. tuberculosis), which is one of the most health-threatening diseases in the world
We found that the Beijing family strains was the most prominent family(81.54%, 243/298)
variable number of tandem repeat (VNTR) results showed that strains were classified into 280 genotypes, and 33 (11.07%) strains could be grouped into 14 clusters. 11 of the 15-VNTR loci were highly or moderately discriminative according to the Hunter-Gaston discriminatory index
Summary
Tuberculosis(TB) is a chronic respiratory infectious diseases caused by Mycobacterium tuberculosis(M. tuberculosis), which is one of the most health-threatening diseases in the world. According to the World Health Organization (WHO)’s report, almost two-thirds of these new cases were in India, China, Indonesia, the Philippines, Pakistan, Nigeria, Bangladesh and South Africa [1]. The WHO estimates that there were about 866,000 new cases of TB patients in China, which has decreased compared with last year, but the epidemic situation of tuberculosis still can’t be ignored. The large population density and high mobility of the population in the middle Shaanxi region are prone to the spread and epidemic risk of tuberculosis to a large extent. The total incidence of TB in Shaanxi province is 56 cases per 100,000 population, according to the Provincial TB Surveillance and Reporting System. Shaanxi Province is experiencing a severe challenge in the TB epidemic
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