Abstract
BackgroundTuberculosis (TB) remains a major global health problem. To investigate the genotypes of Mycobacterium tuberculosis (MTB) and the distribution of Beijing family strains, molecular epidemiology technologies have been used widely.MethodsFrom June 2010 to June 2011, 55 M. tuberculosis isolates from patients with pulmonary TB were studied by Beijing family-specific PCR (detection of the deletion of region of difference 105 [RD105]), and mycobacterial interspersed repetitive units variable number tandem repeat (MIRU-VNTR) analysis. Twenty-four MIRU-VNTR loci defined the genotypes and clustering characteristics of the local strains. All strains were subjected to a drug susceptibility test (DST) by the proportion method on Lowenstein-Jensen (LJ) culture media.ResultsFifty-five clinical isolates of MTB were collected. Beijing family strains represented 85.5% of the isolates studied. Using 24 loci MIRU-VNTR typing categorized the strains into eight gene groups, 46 genotypes, and seven clusters. 83.6% (46/55) of the isolates belonged to the largest gene group. Thirty-six isolates (65.5%) were susceptible, nineteen (34.5%) were resistant to at least one drug, seven (12.8%) were Multidrug-Resistant Tuberculosis (MDR TB), and two (3.6%) were extremely drug-resistant tuberculosis (XDR-TB).ConclusionThe results showed there were obvious polymorphisms of VNTRs of MTB clinical strains. Beijing family strains of MTB were predominant in the Shihezi region of Xinjiang province. There was no correlation between the drug-resistance and Beijing family strains of MTB. It is necessary to strengthen the monitoring, treatment, and management of drug-resistance TB in Shihezi region, Xinjiang.
Highlights
Tuberculosis (TB) remains a major global health problem
Tuberculosis (TB) is an infectious disease caused by the bacillus Mycobacterium tuberculosis (MTB)
By using the 24 loci mycobacterial interspersed repetitive units (MIRU)-variable number of tandem repeats (VNTR) typing, the results showed that 55 strains were categorized into 8 gene groups, 46 genotypes and 7 clusters.83.6% (46/55) isolates belonged to the largest gene group
Summary
Tuberculosis (TB) remains a major global health problem. To investigate the genotypes of Mycobacterium tuberculosis (MTB) and the distribution of Beijing family strains, molecular epidemiology technologies have been used widely. Tuberculosis (TB) is an infectious disease caused by the bacillus Mycobacterium tuberculosis (MTB). TB remains a major public health threat worldwide. China has occupied second place, behind India, among the top five high-burden countries for the last decade In 2010, there were 8.8 million (range, 8.5–9.2 million) incident cases of TB, and 1.1 million (range, 0.9–1.2 million) deaths from TB among HIVnegative people [1]. Genotyping methods have been extensively used to analyze the recent transmission dynamics of MTB.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
