Genotypes and antibiotic resistance of Burkholderia cepacia in Shaoxing area of Zhejiang Province

Abstract

Objective To explore the genotypes of Burkholderia cepacia (BC) and its antibiotic resistance. Methods There were 80 BC complex strains of clinical isolates identified by PCR and sequenced by hisA gene. Using random amplified polymorphic DNA (RAPD) technique to identify genotype of the strains isolated and analyze the homology of the strains. At the same time, drug sensitive tests were conducted among different strains. Results Eighty strains of BC complex were all positive by recA PCR amplification, and the hisA gene sequencing confirmed that the compound was divided into 6 subtypes of which BC was the main type accounting for 85.0% (68 strains) . RAPD technology divided 68 strains of BC into 4 genotypes, including 45 strains of type A (72.6%) , 10 strains of type D, 5 strains of type B and 2 strains of type C. The drug sensitive tests showed that BC type A and D were highly resisted to MIC50 and MIC90 of penicillins, the first to third generation cephalosporins, amino glycosides, imipenem, to which the antibiotic resistance of type A and D were all more than 80.0%. However, type A strain that was locally popular had good sensibility to levofloxacin and trimethoprim/sulfamethoxazole to which the antibiotic resistance rates were 24.4% and 28.9%. Conclusions The major genotype of BC is type A in Shaoxin. Levofloxacin and trimethoprim/sulfamethoxazole can be the first choice to treat BC complex infection clinically. Key words: Burkholderia cepacia complex; Homology; RAPD; Antibiotic resistance