Abstract
Abstract This study was carried out to determine polymorphisms of four genes in South Anatolian Red (SAR) and East Anatolian Red (EAR) indigenous cattle breeds in Turkey. Single nucleotide polymorphisms (SNPs) monitored in this study are Y581S in ATP binding cassette sub family G member 2 (ABCG2) gene, c.1892T>C and c.3359A>C in peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PPARGC1A) gene and g.8232C>A in oxidized low-density lipoprotein receptor 1 (OLR1) gene. The frequency of the ancestral allele A of the ABCG2 gene Y581S polymorphism was found to be very high (SAR: 0.63; EAR: 0.64) in both cattle breeds. The CC genotypes of PPARGC1A gene c.1892T>C (SAR: 0.65; EAR: 0.80) and OLR1 gene g.8232C>A polymorphisms (SAR: 0.82; EAR: 0.86), which are associated with high milk fat percentage, had higher frequencies than those of the other genotypes. In conclusion, we might suggest that the allele distribution of the ABCG2 gene Y581S polymorphism can be the evidence indicating autosomal gene flow from zebu cattle to SAR and EAR cattle breeds.
Highlights
ATP binding cassette sub family G member 2 (ABCG2) gene, which product is expressed in the mammary gland in cows, encodes a transporter protein that facilitates transport of medicines through the cell membrane by binding ATP
Genotype and allele frequencies of polymorphisms Y581S of ABCG2 gene, c.1892T>C and c.3359A>C of PPARGC1A gene, and g.8232C>A of Oxidized Low-density Lipoprotein Receptor 1 (OLR1) gene, which are proposed to have an influence on milk yield parameters, on milk fat percentage, were estimated in South Anatolian Red (SAR) and East Anatolian Red (EAR) cattle breed in Turkey
The most important genes affecting the amount and percentage of milk fat are found on Bos taurus autosomal chromosomes (BTA) 5, 6, 9, 14, 20 and 26 [13]
Summary
ATP binding cassette sub family G member 2 (ABCG2) gene, which product is expressed in the mammary gland in cows, encodes a transporter protein that facilitates transport of medicines through the cell membrane by binding ATP. Acta Veterinaria-Beograd 2014, 64 (1), 73-80 leads to the replacement of the 581th amino acid tyrosine with cysteine [3] Another potential Quantitative Trait Locus (QTL) on the 6th chromosome of cattle is Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1 Alpha (PPARGC1A) gene. It has been reported that one SNP located at 3’UTR region is associated with milk yield traits. This SNP resultes from the translocation of cytosine/timine and results in the replacement of cysteine/alanine at the 223th position in the final protein encoded by this gene [7,8,10]. Genotype and allele frequencies of polymorphisms Y581S of ABCG2 gene, c.1892T>C and c.3359A>C of PPARGC1A gene, and g.8232C>A of OLR1 gene, which are proposed to have an influence on milk yield parameters, on milk fat percentage, were estimated in South Anatolian Red (SAR) and East Anatolian Red (EAR) cattle breed in Turkey
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