Genotoxicity of surface waters in Brazil.

Abstract

Brazil has abundant surface water resources, huge aquatic biodiversity and is home to 213 million people. Genotoxicity assays are sensitive tools to detect the effects of contaminants in surface waters and wastewaters, as well as to determine potential risks of contaminated waters to aquatic organisms and human health. This work aimed to survey the articles published in 2000-2021 that evaluated the genotoxicity of surface waters within Brazilian territory to unveil the profile and trends of this topic over time. In our searches, we considered articles focused on assessing aquatic biota, articles that conducted experiments with caged organisms or standardized tests in the aquatic sites, as well as articles that transported water or sediment samples from aquatic sites to the laboratory, where exposures were performed with organisms or standardized tests. We retrieved geographical information on the aquatic sites evaluated, the genotoxicity assays used, the percentage of genotoxicity detected, and, when possible, the causative agent of aquatic pollution. A total of 248 articles were identified. There was a trend of increase in the number of publications and annual diversity of hydrographic regions evaluated over time. Most articles focused on rivers from large metropolises. A very low number of articles were conducted on coastal and marine ecosystems. Water genotoxicity was detected in most articles, regardless of methodological approach, even in little-studied hydrographic regions. The micronucleus test and the alkaline comet assay were widely applied with blood samples, mainly derived from fish. Allium and Salmonella tests were the most frequently used standard protocols. Despite most articles did not confirm polluting sources and genotoxic agents, the detection of genotoxicity provides useful information for the management of water pollution. We discuss key points to be assessed to reach a more complete picture of the genotoxicity of surface waters in Brazil.