Genotoxicity of meso-2,3-dimercapto succinic acid-coated silver sulfide quantum dot

Abstract

Event Abstract Back to Event Genotoxicity of meso-2,3-dimercapto succinic acid-coated silver sulfide quantum dot Deniz Özkan Vardar1*, Ibrahim Hacıoğlu2, Sevtap Aydin3, Funda Yağcı4 and A. N. Basaran3 1 Hitit University Sungurlu Vocational high Scholl, Department of Health, Türkiye 2 Koç University, Department of Materials Science and Engineering, Türkiye 3 Hacettepe University, Faculty of Pharmacy, Department of Pharmaceutical Toxicology, Türkiye 4 Koç University, Department of Chemistry, Türkiye Nanotecnology products have been used in wide applications in chemistry, electronics, energy generation, and medicine. Despite significant interest in developing quantum dots (QDs) for biomedical applications, many researchers are convinced that QDs will never be used for the treatment of patients because of their potential toxicity. In various in vitro cell culture studies, the cytotoxic properties of some QD have been demonstrated and they have been suggested to be toxic in humans. In this study, the cytotoxic properties of Ag2S-(Meso-2,3-Dimercapto Succinic acid) nanomaterials in V79 cells (Chinese lung fibroblast cell line) were determined by MTT assay. The genotoxic effects of Ag2S-(Meso-2,3-Dimercapto Succinic acid) were evaluated by the alkaline single cell gel electrophoresis. The cells were treated with Ag2S-(Meso-2,3-Dimercapto Succinic acid) at the concentrations of 5- 2000 µg/ml. No cytotoxic effect of Ag2S-(Meso-2,3-Dimercapto Succinic acid) at all concentrations studied was observed. No significant increases in DNA damage were found at the studied concentrations when compared to negative control in V79 cells. In conclusion, further in vitro and in vivo studies are required to determine the safety doses of Ag2S-(Meso-2,3-Dimercapto Succinic acid). Acknowledgements This study was funded in part by a grant from TUBITAK (TUBITAK 3001, contract grant number: 114S861). References Ahamed, M., Karns, M., Goodson, M., Rowe, J., Hussain, S.M., Schlager, J.J., Hong, Y. 2008. “DNA damage response to different surface chemistry of silver nanoparticles in mammalian cells”, Toxicol Appl Pharmacol., 233(3), 404-10. Ahamed, M., Siddiqui, M.A., Akhtar, M.J., Ahmad, I., Pant, A.B., Alhadlaq, H.A. 2010. “Genotoxic potential of copper oxide nanoparticles in human lung epithelial cells”, Biochem Biophys Res Commun., 396(2), 578-83. AshaRani, P.V., Low Kah Mun G., Hande, M.P., Valiyaveettil, S. 2009. “Cytotoxicity and genotoxicity of silver nanoparticles in human cells”, ACS Nano., 3(2), 279-90. Chaung, W. Mi,L.J., Boorstein, R.J. 1997. “The p53 status of Chinese hamster V79 cells frequently used for studies on DNA damage and DNA repair”, Nucleic Acids Res., 25(5), 992-4. Lunov, O., Syrovets, T., Büchele, B., Jiang, X., Röcker, C., Tron, K., Nienhaus, G.U., Walther, P., Mailänder, V., Landfester, K., Simmet, T. 2010. “The effect of carboxydextran-coated superparamagnetic iron oxide nanoparticles on c-Jun N-terminal kinase-mediated apoptosis in human macrophages” Biomaterials, 31(19), 5063-7. Park, E.J., Yi, J., Chung, K.H., Ryu, D.Y., Choi, J., Park, K. 2008. “Oxidative stress and apoptosis induced by titanium dioxide nanoparticles in cultured BEAS-2B cells”, Toxicol Lett., 180(3), 222-9. Keywords: Quantum dots, MTT assay, Comet Assay, Chinese lung fibroblast cell line, Genotoxicity, Nanotoxicity Conference: ICAW 2015 - 11th International Comet Assay Workshop, Antwerpen, Belgium, 1 Sep - 4 Sep, 2015. Presentation Type: Poster Discussion Topic: Genotoxicity testing of chemicals and nanomaterials Citation: Özkan Vardar D, Hacıoğlu I, Aydin S, Yağcı F and Basaran AN (2015). Genotoxicity of meso-2,3-dimercapto succinic acid-coated silver sulfide quantum dot. Front. Genet. Conference Abstract: ICAW 2015 - 11th International Comet Assay Workshop. doi: 10.3389/conf.fgene.2015.01.00018 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 02 Jun 2015; Published Online: 23 Jun 2015. * Correspondence: Mrs. Deniz Özkan Vardar, Hitit University Sungurlu Vocational high Scholl, Department of Health, Çorum, 19300, Türkiye, denizozkantr@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Deniz Özkan Vardar Ibrahim Hacıoğlu Sevtap Aydin Funda Yağcı A. N Basaran Google Deniz Özkan Vardar Ibrahim Hacıoğlu Sevtap Aydin Funda Yağcı A. N Basaran Google Scholar Deniz Özkan Vardar Ibrahim Hacıoğlu Sevtap Aydin Funda Yağcı A. N Basaran PubMed Deniz Özkan Vardar Ibrahim Hacıoğlu Sevtap Aydin Funda Yağcı A. N Basaran Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. 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