Genotoxicity evaluation of Chlorfenapyr in exposed freshwater African catfish Clarias gariepinus using micronucleus test

Imeh Kokoete Esenowo,Akwaowo U Nelson,Okeh C Emmanuel,Chibuisi G Alimba,Ndifreke D Ekpo

doi:10.13170/ajas.6.1.16796

Imeh Kokoete Esenowo, Akwaowo U Nelson + Show 3 more

Open Access

https://doi.org/10.13170/ajas.6.1.16796

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Abstract

Chlorfenapyr is a new formulated insecticide targeted against insecticide resistant species. Its application in targeting harmful aquatic insects may potentially contaminate aquatic environment. This study is to evaluate its genotoxic effects on non-targeted aquatic biota. The study assessed the genotoxic potentials of chlorfenapyr in African catfish (Clarias gariepinus) using the micronucleus (MN) test. Juvenile stage of C. gariepinus were exposed to different concentrations; 5.00 – 15.00 mgL-1 of chlorfenapyr for 96 hours, peripheral blood was collected through the caudal vein and examined for micronucleus induction in the erythrocytes. The tested concentrations of chlorfenapyr did not elicit significant [F (5, 18) = 0.167, p 0.971] increase in MN formation and other nuclear abnormalities in the peripheral erythrocytes of C. gariepinus. The outcome of this study gives credence to the view that chlorfenapyr is not clastogenic and or aneugenic to non-targeted aquatic vertebrate with reference to fish (C. gariepinus).

Highlights

IntroductionThe effects and control of environmental pollution has been a global issue of concern
Over the years, the effects and control of environmental pollution has been a global issue of concern
Juvenile stage of C. gariepinus were exposed to different concentrations; 5.00 – 15.00 mgL-1 of chlorfenapyr for 96 hours, peripheral blood was collected through the caudal vein and examined for micronucleus induction in the erythrocytes

Summary

Introduction

The effects and control of environmental pollution has been a global issue of concern. Ahmed (2007) reported that both animals and humans are victims of the adverse effects of pollution. These adverse effects include several diseases (asthma, cancer), threat to habitat loss, death and extinction of endangered or rare species. Pharmaceuticals and pesticides are common pollutants that contaminates aquatic environments and affecting aquatic life-forms. These contaminants enter the aquatic environment through industrial effluent discharge, agricultural runoff and direct discharge of chemicals into aquatic environment by humans (Muralikrishna and Manickam, 2017)

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