Abstract

The study deals with the genotoxicity of soil samples collected from locations near a thermal power plant in the eastern parts of India. The metal content in the soil samples collected near the thermal power plant, fly ash pond and ash dumping sites were higher than the control sample. Physicochemical parameters such as pH, bulk density, water holding capacity and soil texture did not show much difference between the reference soil and the contaminated soils. Comet assay was carried in pot experiments utilizing Allium bulbs. The results of DNA damage as a reference to the genotoxicity of the soil were positive and could be correlated to the presence of the toxic metal content. The damage was high in the sites located near the ash pond and the ash dumping sites. The root growth was also inhibited in the fly ash contaminated soils. The bioassay data in combination with the physico-chemical analyses will be useful in the management and disposal practices of fly ash and can be undertaken to minimize the adverse impact on agricultural land.

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