Genotoxicity and toxicity assay of water sampled from a radium production industry storage cell territory by means of Allium-test

Abstract

Water from natural reservoirs located near the radium production industry storage cell were analyzed using the anaphase–telophase chromosome aberration assay that was carried out on Allium schoenoprasum L. meristematic root tip cells. 262Ra, 228U, 232Th, 210Pb and 210Po concentrations in all samples were found not to exceed the radioactivity concentration guides. The concentrations of 10 heavy metal ions were measured in water samples, but only Zn and Mn levels exceeded the maximum permissible concentration for the natural reservoirs. All water samples caused a significant increase of the chromosome aberration frequency as compared to control. The chromosome aberration spectrum analysis shows that the genotoxic effect was a result of chemical toxicity mainly. Two samples from the brook springhead were found to be toxic. The regression analysis results show that the mitotic index increased in parallel to Zn ion levels, and decreased with higher 238U concentrations. The water samples genotoxicity positively correlated with the Zn concentration. The present work demonstrates that in order to achieve pollutant screening, it is not sufficient to determine the pollutants concentration only. Adequate conclusions on the risk due to environment contamination need to be based on the additional simultaneous use of toxicity and genotoxicity tests. When bioassays indicate some genotoxic and toxic effects, the determination of the chemical composition of the samples is then required. A combination of these two methods allows the identifcation of the elements that require constant biological monitoring. In the study reported here, those elements are Zn and 238U.