Abstract
The aim of the present study was to assess the genotoxicity, cytotoxicity and immunotoxicity potential of treated wastewater effluents (TWE) discharged from the Vilnius Wastewater Treatment Plant. The analysis of micronuclei, nuclear buds (genotoxicity endpoints), bi-nucleated and fragmentedapoptotic cells (cytotoxicity endpoints) in peripheral blood of rainbow trout (Oncorhynchus mykiss) after a 12- day exposure to TWE revealed a significant increase (p = 0.009) of micronuclei in exposed O. mykiss specimens compared to control fish. No formation of nuclear buds, bi-nucleated and fragmented- apoptotic cells was noted. The analysis of immunotoxicity demonstrated a significant decrease in the neutrophile percentage and an increase in the thrombocyte count in blood of the fish exposed to TWE. The physicochemical analysis of oil products (C14–C28), benzo(a)pyrene and heavy metals (Pb, Cd, Zn, Cu, Hg) in TWE showed that concentrations of xenobiotics satisfy criteria for effluents discharged into receiving wate...
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