Abstract
The toxic effect of strained hydrocarbon 2,2'—bis (bicyclo[2.2.1]heptane) (BBH) was studied using whole-cell bacterial lux-biosensors based on Escherichia coli cells in which luciferase genes are transcriptionally fused with stress-inducible promoters. It was shown that BBH has the genotoxic effect causing bacterial SOS response however no alkylating effect has been revealed. In addition to DNA damage, there is an oxidative effect causing the response of OxyR/S and SoxR/S regulons. The most sensitive to BBH lux-biosensor was E. coli pSoxS-lux which reacts to the appearance of superoxide anion radicals in the cell. It is assumed that the oxidation of BBH leads to the generation of reactive oxygen species, which provide the main contribution to the genotoxicity of this substance.
Highlights
Strained hydrocarbons like norbornane and its non-saturated derivatives are commonly used in the production of rubber, epoxides, medicinal compounds and perfumes [1,2]
Methyl methanesulfonate (MMS) in concentration of 100 μM was used as the alkylating substance in a positive control
DNA damages which are leads to the formation of single-stranded sections of DNA in the cell Oxidation by superoxide anion radicals Total toxicity is measured by the incidence of luminescence, which correlates with the number of living cells
Summary
Strained hydrocarbons like norbornane and its non-saturated derivatives are commonly used in the production of rubber, epoxides, medicinal compounds and perfumes [1,2]. Thermotechnical characteristics of strained hydrocarbons, which have extra internal energy due to deformation of valence bond angles, made them attractive for high-performance combustion applications [3]. Strained 2,2’-bis(bicyclo[2.2.1]heptane) (BBH) compound is a promising as a fuel component for liquid rocket engines. We present the data describing the toxicity of BBH, which we had obtained by utilizing bacterial lux-biosensors.
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