Abstract
Goeckerman therapy is based on combined exposure to UV radiation (UVA, UVB) and crude coal tar (PAHs). Some indicators suggest a genotoxic hazard, however, the level of genotoxic risk of the therapy has not yet been investigated sufficiently. This study aims to assesss the genotoxic risk. The studied group consisted of patients with chronic stable plaque psoriasis treated by Goeckerman therapy (n = 29). Heparin-treated peripheral blood samples were collected one day before the first treatment and immediately after the last procedure. The lymphocytes were isolated from the blood. The level of genotoxicity was evaluated using an alkaline version of the Comet assay which detects DNA single strand breaks (DNA-SSBs), a neutral version of the Comet assay which detects DNA double strand breaks (DNA-DSBs), and using chromosomal aberrations. The level of DNA-SSBs increased insignificantly (median; Q1-Q3): 1.4 (0.4; 0.1-1.4) vs. 2.5 (0.6; 0.3-2.7) %tDNA (P = 0.11) and the level of DNA-DSBs increased significantly: 7.8 (6.5; 3.4-10.5) vs. 20.7 (19.3; 14.2-24.6) % DNA (P < 0.001). The total number of aberrated cells (P < 0.001) and structurally aberrated cells (P < 0.001) increased significantly. The elevated levels of the DNA-DSBs and the chromosomal aberrations in the peripheral lymphocytes indicated a genotoxic hazard. However, the elevated level of the chromosomal abnormalities was below the upper level of the reference range for healthy Czech adults. While, the genotoxic risk appears to be low, Goeckerman treatment represents a further contribution to the lifetime load of genotoxic factors.
Highlights
The elevated level of the chromosomal abnormalities was below the upper level of the reference range for healthy Czech adults
The genotoxic risk appears to be low, Goeckerman treatment represents a further contribution to the lifetime load of genotoxic factors
The group consisted of patients with chronic stable plaque psoriasis treated by Goeckerman therapy (GT) (Clinic of Dermal and Venereal Diseases, University Hospital, Hradec Kralove, Czech Republic)
Summary
The studied group consisted of patients with chronic stable plaque psoriasis treated by Goeckerman therapy (n = 29). The level of genotoxicity was evaluated using an alkaline version of the Comet assay which detects DNA single strand breaks (DNA-SSBs), a neutral version of the. The group consisted of patients with chronic stable plaque psoriasis treated by GT (Clinic of Dermal and Venereal Diseases, University Hospital, Hradec Kralove, Czech Republic). The lower boundary of the box: 1st quartile (Q1). The upper boundary of the box: 3rd quartile (Q3). The lower whisker boundary: the smallest observation that is greater than or equal to Q1 – 1.5 x IQR. The upper whisker boundary: the largest observation that is less than or equal to Q3 + 1.5 x IQR. We measured structurally aberrated cells, numerically aberrated cells and total number of aberrated cells
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