Genotoxic and hepatic biotransformation responses induced by the overflow of pulp mill and secondary-treated effluents on Anguilla anguilla L.

Abstract

Pulp and paper mill effluent compounds pollute the aquatic environment and are responsible for increased biochemical alterations and genotoxicity in aquatic organisms such as fish. Adult eels ( Anguilla anguilla L) were exposed during 8, 16, 24, and 72 h to the following conditions: (1) aerated, filtered, and dechlorinated tap water ( C); (2) 2.5% (v/v) sewage water previously treated with activated sludge ( T); (3) bleached kraft pulp and paper mill effluent collected at the river Vouga, close to an ancient sewage outlet (Portucel), diluted in tap water [25% ( E 25 ) and 50% ( E 50 )]; and (4) bleached kraft pulp and paper mill effluent sediment [water-soluble fraction ( S)]. Liver biotransformation induced by the above conditions was measured as ethoxyresorufin- O-deethylase (EROD), cytochrome P450 (P450) (Phase I), and glutathione- S-transferase (GST) (Phase II). Genotoxicity was also determined as blood/liver DNA strand breaks and erythrocytic nuclear abnormalities (ENA) induced on European eel ( A. anguilla L). Liver EROD activity was significantly increased in eels at 8 and 16 h exposure to E 25 , as well as at 16, 24, and 72 h exposure to E 50 . S exposure induced liver EROD activity only at 24 h. A significant decrease in liver P450 was observed at 72 h exposure to T, whereas a significant P450 increase at 16 h was followed by a significant decrease at 24 h exposure to E 25 . Another P450 significant increase was noticed at 72 h exposure to S. Liver GST activity (Phase II) demonstrated a significant increase at 72 h exposure to E 50 and to S. A significant decrease in blood DNA integrity was observed at 72 h exposure to T and at 24 and 72 h to S. Blood DNA integrity significantly decreased at 16 and 24 h exposure to E 25 , as well as at 8, 16, and 24 h exposure to E 50 . Liver DNA integrity significantly decreased at 72 h exposure to T and at 16 h exposure to S. Moreover , liver DNA integrity was significantly decreased at 24 h exposure to E 25 and E 50 , and 72 h to E 50 . A. anguilla L. increased ENA frequency was detected in T at 16, 24, and 72 h, whereas in E 25 and S it was observed at 8, 16, and 24 h. Furthermore, E 50 ENA frequency increased at 24 h exposure.