Abstract
Cerrena unicolor is an ecologically and biotechnologically important wood-degrading basidiomycete with high lignocellulose degrading ability. Biological and genetic investigations are limited in the Cerrena genus and, thus, hinder genetic modification and commercial use. The aim of the present study was to provide a global understanding through genomic and experimental research about lignocellulosic biomass utilization by Cerrena unicolor. In this study, we reported the genome sequence of C. unicolor SP02 by using the Illumina and PacBio 20 platforms to obtain trustworthy assembly and annotation. This is the combinational 2nd and 3rd genome sequencing and assembly of C. unicolor species. The generated genome was 42.79 Mb in size with an N50 contig size of 2.48 Mb, a G + C content of 47.43%, and encoding of 12,277 predicted genes. The genes encoding various lignocellulolytic enzymes including laccase, lignin peroxidase, manganese peroxidase, cytochromes P450, cellulase, xylanase, α-amylase, and pectinase involved in the degradation of lignin, cellulose, xylan, starch, pectin, and chitin that showed the C. unicolor SP02 potentially have a wide range of applications in lignocellulosic biomass conversion. Genome-scale metabolic analysis opened up a valuable resource for a better understanding of carbohydrate-active enzymes (CAZymes) and oxidoreductases that provide insights into the genetic basis and molecular mechanisms for lignocellulosic degradation. The C. unicolor SP02 model can be used for the development of efficient microbial cell factories in lignocellulosic industries. The understanding of the genetic material of C. unicolor SP02 coding for the lignocellulolytic enzymes will significantly benefit us in genetic manipulation, site-directed mutagenesis, and industrial biotechnology.
Highlights
C. unicolor has long been a traditional Chinese medicinal fungus and has been widely utilized to treat many human ailments in Asian countries
The selected isolate coded as SP02 was identified based on its morphology and the ITS-5.8S ribosomal gene sequence method
It was observed that the isolate developed a pure white mat with typical concentric zones in Petri dish cultures
Summary
C. unicolor has long been a traditional Chinese medicinal fungus and has been widely utilized to treat many human ailments in Asian countries. C. unicolor has a unique capacity to degrade recalcitrant resistant lignin polymers as well as a wide spectrum of aromatic pollutants that provide potential value in biomass utilization and organo-pollutant degradation [10,11]. Selective delignification by white-rot fungi like C. unicolor preferentially removes lignin from wood and leaves pockets of white degraded cells that consist entirely of cellulose [13,14], providing an eco-friendly way to obtain the high-value products and recycle the excess of agro-industrial waste [15]. In the context of C. unicolor’s abilities to degrade lignocellulosic biomass and production of biotechnologically significant compounds, better understanding of the inner relationship of lignocellulolytic enzymes and selective substrate utilize mechanisms may be extremely valuable
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