Genomic Structure and Expression Sites of Three Gonadotropin-Releasing Hormone Genes in One Species

Abstract

In the teleost fish,Haplochromis burtoni,gonadotropin-releasing hormone (GnRH) peptide has been localized to three distinct regions in the brain. Each GnRH population is associated with expression of a distinct cDNA as previously described. Here we report the complete genomic sequences encoding these three forms and compare their structural organization, putative regulatory elements, and expression patterns in the body. All three genes share a common structure of four exons: the first exon encodes the 5′ untranslated region; the second exon encodes the signal sequence, GnRH decapeptide, and the 5′ end of the GnRH-associated peptide (GAP); the third exon consists entirely of GAP coding sequence; and the fourth exon encodes the 3′ end of GAP and the 3′ untranslated region. Each of the three GnRH genes has been shown previously to have a distinct spatial expression pattern in the brain, and here we use reverse transcription and cDNA amplification to demonstrate that each gene is expressed in the body. The gene encoding the releasing form, {Ser8}GnRH, is expressed in the heart, liver, spleen, kidney, and testis, as well as in the preoptic area. The {His5Trp7Tyr8}GnRH gene is expressed in the testis as well as in the midbrain. The {Trp7Leu8}GnRH gene is expressed in the testis and the terminal nerve area. We examined the 500 bp upstream of exon 1 in all threeH. burtonigenes and identified putative binding sites for glucocorticoid receptor, androgen receptor, and progesterone receptor, as well as the transcription factors Ap-1 and Sp-1. The genomic sequence encoding the terminal nerve form of GnRH (i.e., {Trp7Leu8}GnRH) inH. burtoniis remarkably similar to that encoding the presumed releasing form of GnRH in salmonids, especially in the 3′ intergenic region. Taken together with phylogenetic and mRNA localization data in salmonids, these data suggest that the gene encoding the releasing form of GnRH in salmonids may not yet be described.