Abstract
The complete nucleotide sequence of Sucra jujuba nucleopolyhedrovirus (SujuNPV) was determined by 454 pyrosequencing. The SujuNPV genome was 135,952 bp in length with an A+T content of 61.34%. It contained 131 putative open reading frames (ORFs) covering 87.9% of the genome. Among these ORFs, 37 were conserved in all baculovirus genomes that have been completely sequenced, 24 were conserved in lepidopteran baculoviruses, 65 were found in other baculoviruses, and 5 were unique to the SujuNPV genome. Seven homologous regions (hrs) were identified in the SujuNPV genome. SujuNPV contained several genes that were duplicated or copied multiple times: two copies of helicase, DNA binding protein gene (dbp), p26 and cg30, three copies of the inhibitor of the apoptosis gene (iap), and four copies of the baculovirus repeated ORF (bro). Phylogenetic analysis suggested that SujuNPV belongs to a subclade of group II alphabaculovirus, which differs from other baculoviruses in that all nine members of this subclade contain a second copy of dbp.
Highlights
Baculoviruses are rod-shaped, insect-specific viruses with double-stranded, circular DNA 80–180 kb genomes [1]
Gene parity plots were generated in order to analyze the gene order of Sucra jujuba nucleopolyhedrovirus (SujuNPV) relative to three other closely related baculoviruses (ApciNPV, Ectropis obliqua NPV (EcobNPV) and Orgyia leucostigma NPV (OrleNPV)) and the five representative viruses mentioned above
131 putative open reading frames (ORFs) were detected in the SujuNPV genome with the criteria of a length of at least 50 amino acids and a minimal overlap with adjacent ORFs
Summary
Baculoviruses are rod-shaped, insect-specific viruses with double-stranded, circular DNA 80–180 kb genomes [1]. Baculoviruses have been widely used as bio-pesticides to control insect pests in agriculture and forestry [2], as vectors for protein expression, and as potential vectors for gene therapy [3,4]. The family Baculoviridae used to be grouped into two genera: Nucleopolyhedroviruses (NPVs) and Granuloviruses (GVs), dependent upon differing morphologies of occlusion bodies (OBs). A new classification has subdivided the Baculoviridae into four genera, based on phylogeny and host specificities: Alphabaculovirus (lepidopteran-specific NPVs), Betabaculovirus (lepidopteran-specific GVs), Gammabaculovirus (hymenopteran-specific NPVs) and Deltabaculovirus (dipteranspecific NPVs) [6]. 62 baculovirus reference genomes are available in the National Centre for Biotechnology Information (NCBI) database; 42 of them are alphabaculoviruses, 15 betabaculoviruses, three gammabaculoviruses, one deltabaculovirus and one unclassified baculovirus
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