Genomic Sequence of the New Attenuated Vaccine Strain Neethling-RIBSP of the Lumpy Skin Disease Virus.

M B Orynbayev,A M Melisbek,R K Nissanova,B S Myrzakhmetova,A Shevtsov,T U Argimbayeva,S M Barmak,K T Sultankulova,A K Nakhanov,N S Kozhabergenov,A U Issabek,K D Zakarya,Jelle Matthijnssens

doi:10.1128/mra.00318-20

Abstract

We report here the draft genome sequence of the new attenuated strain Neethling-RIBSP of the lumpy skin disease virus, obtained by sequential and alternating passages in cell culture and developing chicken embryos. Genome sequencing allowed the identification of differentiation markers of the new strain.

Highlights

Lumpy skin containing disease is virus of the an infectious Capripoxvirus disease of viral genus within the etiology caused Poxviridae family by (1)
The longest contig, with a coverage of 59.5ϫ, was identified as lumpy skin disease virus, and the remaining contigs were assigned to Bos taurus
A pairwise comparison analysis showed that the new vaccine strain is 99.96% identical to the Kubash/KAZ/16 strain

Summary

Introduction

Lumpy skin containing disease is virus of the an infectious Capripoxvirus disease of viral genus within the etiology caused Poxviridae family by (1). The current genome characterization will allow us to better understand the attenuation mechanism for obtaining vaccine strains and reveal mutations for their differentiation from the other strains. We report a near-complete genome sequence of the Neethling-RIBSP strain. DNA was extracted from a virus purified on a stepwise sucrose density gradient of 45 to 48 to 60% (4) using the DNeasy blood and tissue kit 250) (Qiagen) following the manufacturer’s protocol.

Results

Conclusion