Abstract
The comber alphavirus was isolated from a fish cell line from the brain of an apparently healthy Serranus cabrilla specimen collected during wild fish surveillance in southern Italy. The comber alphavirus is a new member of the genus Alphavirus, family Togaviridae.
Highlights
Alphavirus ruses are is the only enveloped genus presently included in the Togaviridae family. single-stranded positive-sense RNA genomes ranging
Virions were precipitated from BF-2 cell supernatant with polyethylene glycol and purified using ultracentrifugation on a 40 to 70% sucrose gradient to obtain a visible viral band, which was diluted in 10 ml of TRIS-HCl-EDTA-NaCl (TEN) buffer, pelleted by ultracentrifugation, and, resuspended in 1 ml of TEN buffer (6)
Genomic sequence of a new Alphavirus detected in comber (Serranus cabrilla)
Summary
Alphavirus ruses are is the only enveloped genus presently included in the Togaviridae family. single-stranded positive-sense RNA genomes ranging. The new virus was called comber alphavirus isolate 12ITT-210.14. RNA was extracted using the total RNA isolation kit NucleoSpin RNA II (Macherey-Nagel) and converted into first- and second-strand cDNA using SuperScript III reverse transcriptase (Invitrogen) and second-strand cDNA synthesis (NEB), respectively.
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