Abstract
A series of human and animal diseases that are caused by Salmonella infections pose a serious threat to human health and huge economic losses to the livestock industry. We found antibiotic resistance (AR) genes in the genome of 133 strains of S. Indiana from a poultry production site in Shandong Province, China. Salmonella enterica subsp. enterica serovar Indiana strain MHYL had multidrug-resistance (MDR) genes on its genome. Southern blot analysis was used to locate genes on the genomic DNA. High-throughput sequencing technology was used to determine the gene sequence of the MHYL genome. Areas containing MDR genes were mapped based on the results of gene annotation. The AR genes blaTEM, strA, tetA, and aac(6′)-Ib-cr were found on the MHYL genome. The resistance genes were located in two separate MDR regions, RR1 and RR2, containing type I integrons, and Tn7 transposons and multiple IS26 complex transposons with transposable functions. Portions of the MDR regions were determined to be highly homologous to the structure of plasmid pAKU_1 in S. enterica serovar Paratyphi A (accession number: AM412236), SGI11 in S. enterica serovar Typhimurium (accession number: KM023773), and plasmid pS414 in S. Indiana (accession No.: KC237285).
Highlights
Salmonella species are a major worldwide bacterial cause of acute gastroenteritis [1,2]
The results showed that each drug-resistance gene was successfully hybridized and mapped to the strain MHYL genomic DNA both before and after digestion
The electrophoresis map of the strain MHYL genomic DNA and the hybridization results for each drug-resistance gene were compared, and the band sizes were consistent
Summary
Salmonella species are a major worldwide bacterial cause of acute gastroenteritis [1,2]. Salmonellosis is commonly caused by non-typhoidal Salmonella enterica serotypes [2]. Salmonella enterica serovar Indiana was first reported in China during 1984 and is commonly found in animals, animal processing facilities, food, and people [5]. There have been reports of human infection with diarrhea caused by multidrug-resistant S. Through a study of multiple-chromosomal-type resistant Salmonella, it was determined that most of these resistance genes were concentrated in a small genomic island called SGI1 (Salmonella genomic island 1), and the area on the genome where these resistance genes are located is called the MDR region [9]. Due to the mobility of the resistance genes in SGI1, it is of great significance when studying the acquisition and transmission of bacterial MDR. Gene replacement in the integron structure is another way to contribute to the variability of the SGI1 antibiotic resistance (AR) gene cluster, which may result in stronger virulence and the further exchange of AR genes between other multidrug-resistant bacteria [12]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
