Abstract
Voles of the genus Microtus are important research organisms, yet genomic resources are lacking. Such resources would benefit future studies of immunology, phylogeography, cryptic diversity, and more. We sequenced and assembled nuclear genomes from two subspecies of water vole (Microtus richardsoni) and from the montane vole (Microtus montanus). The water vole genomes were sequenced with Illumina and 10× Chromium plus Illumina sequencing, resulting in assemblies with ∼1600,000 and ∼30,000 scaffolds, respectively. The montane vole was also assembled into ∼13,000 scaffolds using Illumina sequencing. Mitochondrial genome assemblies were also performed for both species. Structural and functional annotation for the best water vole nuclear genome resulted in ∼24,500 annotated genes, with 83% of these having functional annotations. Assembly quality statistics for our nuclear assemblies fall within the range of genomes previously published in the genus Microtus, making the water vole and montane vole genomes useful additions to currently available genomic resources.
Highlights
The genus Microtus comprises 62 species of voles, distributed throughout North America, Europe, and Asia [1]
Assembled genomes for four Microtus species have been deposited in GenBank: two European species (M. agrestis and M. arvalis) and two North American species (M. ochrogaster; [9], and M. oeconomus)
The present study provides resources for two additional species: M. richardsoni (NCBI:txid111840) and M. montanus (NCBI:txid88450)
Summary
The genus Microtus comprises 62 species of voles, distributed throughout North America, Europe, and Asia [1]. We present single nuclear and mitochondrial genome assemblies for M. montanus. Owing to low sequencing coverage, de novo genome assembly was not appropriate for M. montanus. Arvicoloides produced over 800 million reads and 47× genome sequencing coverage.
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