Abstract
A Gram-positive, acid-fast and rapidly growing rod, designated S2-37T, that could form yellowish colonies was isolated from one soil sample collected from cotton cropping field located in the Xinjiang region of China. Genomic analyses indicated that strain S2-37T harbored T7SS secretion system and was very likely able to produce mycolic acid, which were typical features of pathogenetic mycobacterial species. 16S rRNA-directed phylogenetic analysis referred that strain S2-37T was closely related to bacterial species belonging to the genus Mycolicibacterium, which was further confirmed by pan-genome phylogenetic analysis. Digital DNA-DNA hybridization and the average nucleotide identity presented that strain S2-37T displayed the highest values of 39.1% (35.7-42.6%) and 81.28% with M. litorale CGMCC 4.5724T, respectively. And characterization of conserved molecular signatures further supported the taxonomic position of strain S2-37T belonging to the genus Mycolicibacterium. The main fatty acids were identified as C16:0, C18:0, C20:3ω3 and C22:6ω3. In addition, polar lipids profile was mainly composed of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. Phylogenetic analyses, distinct fatty aids and antimicrobial resistance profiles indicated that strain S2-37T represented genetically and phenotypically distinct from its closest phylogenetic neighbour, M. litorale CGMCC 4.5724T. Here, we propose a novel species of the genus Mycolicibacterium: Mycolicibacterium gossypii sp. nov. with the type strain S2-37T (= JCM 34327T = CGMCC 1.18817T).
Highlights
The genus Mycolicibacterium presents the second largest population of Gram-positive, acidfast and rod-shaped microbes in the family Mycobacteriaceae (Parte et al 2020)
At the time of writing, the genus Mycolicibacterium is composed of 92 recognized species with published names (Parte et al 2020), which primarily harbors rapid-growing species isolated from diverse range of environments, including river water, marine sediment and soil (Butler et al 1993; Brown-Elliott et al 2010; Zhang et al 2013)
It is well documented that mycobacterial species displayed relatively high diverses in their genomic features at species level (Fedrizzi et al 2017), which has already remarkably enhanced the difficulty accurately characterizing the taxonomic position of microbial species belonging to this population
Summary
The genus Mycolicibacterium presents the second largest population of Gram-positive, acidfast and rod-shaped microbes in the family Mycobacteriaceae (Parte et al 2020). RRNA gene sequence of the strain S2-37 T is MW295419. Multilocus sequence analysis of three genes (16S rRNA, hsp65 and rpoB) presented that strain S2-37T
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
