Abstract
Here, we report the full-genome sequence of an NAD-hemin-independent Avibacterium paragallinarum serovar C-2 strain, FARPER-174, isolated from layer hens in Peru. This genome contained 12 potential genomic islands that include ribosomal protein-coding genes, a nadR gene, hemocin-coding genes, sequences of fagos, an rtx operon, and drug resistance genes.
Highlights
Avibacterium paragallinarum is the etiologic agent of infectious coryza, an acute respiratory disease of chickens, which is globally distributed and causes serious economic losses in the poultry production industry
DNA was extracted from the fresh bacterial culture (500 ml of brain heart infusion (BHI) without factors at 37°C with agitation at 180 rpm for 24 hours) centrifuged at 5,000 ϫ g for 15 min
The genome was sequenced on the PacBio RS II platform (Pacific Biosciences, CA) using P6-C4 chemistry and assembled with Hierarchical Genome Assembly Process (HGAP) version 3.0 [16] with default parameters by Macrogen, Inc. (South Korea)
Summary
Avibacterium paragallinarum is the etiologic agent of infectious coryza, an acute respiratory disease of chickens, which is globally distributed and causes serious economic losses in the poultry production industry. DNA was extracted from the fresh bacterial culture (500 ml of BHI without factors at 37°C with agitation at 180 rpm for 24 hours) centrifuged at 5,000 ϫ g for 15 min. The pellet was resuspended in phosphate-buffered saline (PBS) for DNA isolation using the phenol-chloroform protocol [15]. The genome was sequenced on the PacBio RS II platform (Pacific Biosciences, CA) using P6-C4 chemistry and assembled with Hierarchical Genome Assembly Process (HGAP) version 3.0 [16] with default parameters by Macrogen, Inc.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
