Abstract
Here, we report the full-genome sequence of an NAD-hemin-independent Avibacterium paragallinarum serovar C-2 strain, FARPER-174, isolated from layer hens in Peru. This genome contained 12 potential genomic islands that include ribosomal protein-coding genes, a nadR gene, hemocin-coding genes, sequences of fagos, an rtx operon, and drug resistance genes.
Highlights
Avibacterium paragallinarum is the etiologic agent of infectious coryza, an acute respiratory disease of chickens, which is globally distributed and causes serious economic losses in the poultry production industry
DNA was extracted from the fresh bacterial culture (500 ml of brain heart infusion (BHI) without factors at 37°C with agitation at 180 rpm for 24 hours) centrifuged at 5,000 ϫ g for 15 min
The genome was sequenced on the PacBio RS II platform (Pacific Biosciences, CA) using P6-C4 chemistry and assembled with Hierarchical Genome Assembly Process (HGAP) version 3.0 [16] with default parameters by Macrogen, Inc. (South Korea)
Summary
Avibacterium paragallinarum is the etiologic agent of infectious coryza, an acute respiratory disease of chickens, which is globally distributed and causes serious economic losses in the poultry production industry. DNA was extracted from the fresh bacterial culture (500 ml of BHI without factors at 37°C with agitation at 180 rpm for 24 hours) centrifuged at 5,000 ϫ g for 15 min. The pellet was resuspended in phosphate-buffered saline (PBS) for DNA isolation using the phenol-chloroform protocol [15]. The genome was sequenced on the PacBio RS II platform (Pacific Biosciences, CA) using P6-C4 chemistry and assembled with Hierarchical Genome Assembly Process (HGAP) version 3.0 [16] with default parameters by Macrogen, Inc.
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