Genomic in situ hybridization identifies parental chromosomes in hybrid scallop (Bivalvia, Pectinoida, Pectinidae) between female Chlamysfarreri and male Argopectenirradiansirradians.

Lingling Zhang,Ke Bi,Xiaoting Huang,Zhenmin Bao,Shi Wang,Wei Lu

doi:10.3897/compcytogen.v9i2.8943

Abstract

Interspecific crossing was artificially carried out between Chlamys farreri (Jones & Preston, 1904) ♀ and Argopecten irradians irradians (Lamarck, 1819) ♂, two of the dominant cultivated scallop species in China. Genomic in situ hybridization (GISH) was used to examine the chromosome constitution and variation in hybrids at early embryonic stage. The number of chromosomes in 66.38% of the metaphases was 2n = 35 and the karyotype was 2n = 3 m + 5 sm + 16 st + 11 t. After GISH, two parental genomes were clearly distinguished in hybrids, most of which comprised 19 chromosomes derived from their female parent (Chlamys farreri) and 16 chromosomes from their male parent (Argopecten irradians irradians). Some chromosome elimination and fragmentation was also observed in the hybrids.

Highlights

Utilization of heterosis has become one of the most important strategies for increasing productivity of commercial plants and animals (Hua et al 2003)
The other is reciprocal hybrid between Argopecten irradians irradians (Lamarck, 1819) and A. purpuratus (Lamarck, 1819), and the hybrids exhibited a great increase in production traits as well as some interesting new characteristics (Wang et al 2011)
We reported experimental results of using Genomic in situ hybridization (GISH) to verify the hybrid identity of the larvae, and documented a number of interesting patterns of karyotypic abnormalities in some hybrids

Summary

Introduction

Utilization of heterosis has become one of the most important strategies for increasing productivity of commercial plants and animals (Hua et al 2003). Lü et al (2006a and 2006b) reported that the chromosome number in most of the scallop hybrid between C. farreri and P. yessoensis was 38, which was accordant to that of their parents. Most of the hybrid metaphase plates had a diploid chromosome number of 35, as expected from the parental haploid complements.

Results

Conclusion