Abstract
Using a simple metagenomic approach, we identified a divergent human parechovirus (HPeV) in the stool of a child in Pakistan. Genomic characterization showed this virus was distinct enough from reported HPeV types to qualify as candidate prototype for the seventh HPeV type.
Highlights
Using a simple metagenomic approach, we identified a divergent human parechovirus (HPeV) in the stool of a child in Pakistan
More severe consequences have been ascribed to HPeV infections, including acute flaccid paralysis (AFP) [3], encephalitis [8], aseptic meningitis [9], myocarditis [10], neonatal sepsis [11], and Reye syndrome [6], Nonpolio AFP may be caused by many viruses, including nonpolio enteroviruses, human adenoviruses, herpes simplex virus, Epstein-Barr virus, and West Nile virus [12]
Of the 6 known HPeV types, the intertype amino acid identities ranged from 84.9% to 91.1%, and the intertype nucleotide identities of the open reading frame (ORF) sequence ranged from 76.1% to 83.4%, a range similar to their identities relative to PAK5045
Summary
Using a simple metagenomic approach, we identified a divergent human parechovirus (HPeV) in the stool of a child in Pakistan. Genomic characterization showed this virus was distinct enough from reported HPeV types to qualify as candidate prototype for the seventh HPeV type. Using sequence-independent PCR amplification and sequence similarity searches, we recently investigated virus sequences in stool samples from children in Pakistan who had nonpolio AFP and from healthy children who had close contacts with persons who had AFP. A highly divergent HPeV type was identified in 1 contact sample, and the full genome of this virus was sequenced. Comparison of the complete ORF of PAK5045 with the 6 HPeV prototypes showed it was closely related to HPeVs and had amino acid
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