Abstract

Canine circovirus (CanineCV) was detected, together with canine parvovirus (CPV), in samples from an outbreak of fatal gastroenteritis in dogs in Argentina. We obtained the full-length genome of this recently discovered virus by overlapping PCR, designated strain UBA-Baires. Sequence analysis revealed a highly conserved genome but also showed several unique mutations in amino acids from the capsid protein that have not been previously reported. Phylogenetic analysis shows that this strain is more closely related to European strains than to viruses detected in North America or Asia. Although the pathogenic role of CanineCV in dogs is still unclear, this study highlights the importance of CanineCV as a coinfecting virus in disease development. To our knowledge, this is the first report of the involvement of CanineCV in severe clinical disease in dogs in South America. Our results expand our information on the geographical extent of this virus and contribute to the understanding of its role in disease.

Highlights

  • A newly described canine circovirus (CanineCV) was identified recently in serum from healthy dogs [1]

  • We report an outbreak of a fatal disease associated with the presence of CanineCV in Argentina

  • We found only one unique mutation in the rep gene, but surprisingly, four unique aa substitutions in the capsid protein (Cap) protein, after two independent experiments using different sequencing facilities

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Summary

Introduction

A newly described canine circovirus (CanineCV) was identified recently in serum from healthy dogs [1]. Several countries have reported the molecular detection of CanineCV in cases associated with clinical disease, ranging from sudden death of puppies with bloody diarrhea to respiratory syndromes [2,3,4,5,6]. A number of case-control studies have been published, most of them describing significant association between diarrhea and CanineCV detection [8,9,10,11].

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