Abstract
BackgroundThe blaNDM-1 gene in Salmonella species is mostly reported in clinical cases, but is rarely isolated from red and white meat in China.MethodsA Salmonella Indiana (S. Indiana) isolate was cultured from a chicken carcass procured from a slaughterhouse in China. Antimicrobial susceptibility was tested against a panel of agents. Whole-genome sequencing of the isolate was carried out and data was analyzed.ResultsA large plasmid, denoted as plasmid pC629 (210,106 bp), containing a composite cassette, consisting of IS26-blaNDM-1-bleMBL-△trpF-tat-cutA-ISCR1-sul1-qacE△1-aadA2-dfrA12-intI1-IS26 was identified. The latter locus was physically linked with blaOXA-1, blaCTX-M-65, blaTEM-1-encoding genes. A mercury resistance operon merACDEPTR was also identified; it was flanked on the proximal side, among IS26 element and the distally located on the blaNDM-1 gene. Plasmid pC629 also contained 21 other antimicrobial resistance-encoding genes, such as aac(6′)-Ib-cr, aac(3)-VI, aadA5, aph(4)-Ia, arr-3, blmS, brp, catB3, dfrA17, floR, fosA, mph(A), mphR, mrx, nimC/nimA, oqxA, oqxB, oqxR, rmtB, sul1, sul2. Two virulence genes were also identified on plasmid pC629.ConclusionTo the best of our knowledge, this is the first report of blaNDM-1 gene being identified from a plasmid in a S. Indiana isolate cultured from chicken carcass in China.
Highlights
The blaNDM-1 gene in Salmonella species is mostly reported in clinical cases, but is rarely isolated from red and white meat in China
The New Delhi metallo-beta-lactamase (NDM)-1 encoding gene was first detected in Klebsiella pneumoniae recovered from a Swedish patient who was infected with an antibiotic-resistant bacterium acquired in New Delhi, India [2, 3]
Indiana C629 isolate was only susceptible to colistin (MIC 0.5 mg/L) and resistant to 18 compounds representing 10 different antimicrobial classes (Table 1)
Summary
The blaNDM-1 gene in Salmonella species is mostly reported in clinical cases, but is rarely isolated from red and white meat in China. The New Delhi metallo-beta-lactamase (NDM) is one of the most commonly reported carbapenemase resistance mechanisms in the world [1]. The NDM-1 encoding gene (blaNDM-1) was first detected in Klebsiella pneumoniae recovered from a Swedish patient who was infected with an antibiotic-resistant bacterium acquired in New Delhi, India [2, 3]. Thereafter, this plasmid-mediated NDM-1 resistance mechanism has been widely reported [4].
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