Abstract
Dendrobium officinale Kimura et Migo is of great importance as a traditional Chinese herb due to its abundant metabolites. The family of basic helix-loop-helix (bHLH) transcription factors widely exists in plants and plays an essential role in plant growth and development, secondary metabolism as well as responses to environmental changes. However, there is limited information on bHLH genes in D. officinale. In the present study, a total of 98 putative DobHLH genes were identified at the genomic level, which could be classified into 18 clades. Gene structures and conserved motifs in DobHLH genes showed high conservation during their evolution. The conserved amino acids and DNA bindings of DobHLH proteins were predicted, both of which are pivotal for their function. Furthermore, gene expression from eight tissues showed that some DobHLH genes were ubiquitously expressed while other DobHLH genes were expressed in the specific tissues. Expressional changes of DobHLH genes under MeJA and ABA treatments were detected by qRT-PCR. The protein–protein interactions between DobHLHs were predicted and several interactions were confirmed by yeast two hybrid. Therefore, our results here contribute to the understanding of bHLH genes in D. officinale and lay a foundation for the further functional study of its biological processes.
Highlights
Dendrobium officinale Kimura et Migo is a perennial herbal plant, belonging to the family Orchidaceae
They were named from DobHLH1 to DobHLH98 according to their relationship with
The subcellular localization of 98 DobHLH proteins were predicted by WOLF
Summary
Dendrobium officinale Kimura et Migo (or known as D. catenatum) is a perennial herbal plant, belonging to the family Orchidaceae. D. officinale is of great importance due to its medicinal and ornamental uses. D. officinale ranks first among the nine Chinese herbs for longevity and has a documented use in folk medicine for over 1300 years [2]. About 190 compounds have been identified and isolated from D. officinale plants including phenanthrenes, bibenzyls, saccharides and glycosides, alkaloids, essential oils, and others such as phenols, acids, esters, and amides [3]. The abundance of polysaccharides in its stems and the specific alkaloids make D. officinale have anticancer [1], anti-oxidant [5], and anti-inflammatory effects [1,6] as well as support immune modulation [7,8], hepatop protection, Plants 2020, 9, 1044; doi:10.3390/plants9081044 www.mdpi.com/journal/plants
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