Genomic approach to identify association of environmental bisphenol-A (BPA) in daily use plastics as molecular disruptors in breast cancer

Abstract

PurposeThe present study was aimed to investigate the association of Bisphenol-A (BPA) and MDM2 SNP309 polymorphism with the risk of breast cancer in Indian women. MethodsBlood samples from breast cancer patients and healthy controls were analyzed by using UV visible spectrophotometry for structural changes in blood components by BPAQ. MDM2 SNP309 polymorphism was investigated by using TETRA ARMS-PCR. ResultsOn comparing control groups, there is an increased mean absorbance at a range of 250 nm to 300 nm and 360 nm to 450 nm in breast cancer patients. The allele frequencies of case subjects (T, 0.47; G, 0.53) were significantly different from those of control subjects (T, 0.66; G, 0.34) (p = 0.011). The proportion of GG genotype of the SNP309 in breast cancer patients (28.88%) was significantly higher than that of control groups (13.33%). With TT genotype as reference, the genotypes (TG + GG) containing G allele as dominant form in breast cancer patients (OR, 2.80; 95% CI, 1.12–7.00); p = 0.025) were significantly associated with breast cancer. ConclusionsThe results revealed that there is an association between active metabolite of Bisphenol-A and MDM2 SNP309 G variant with the risk of breast cancer in Indian population.