Abstract
Genomic data together with sequencing of tissue specific small RNA libraries reveals insights into the genome content, small RNA repertoire and evolutionary origins of the grass Miscanthus × giganteus.
Highlights
Miscanthus × giganteus (Mxg) is a perennial grass that produces superior biomass yields in temperate environments
Genome size estimation for Miscanthus species We used flow cytometry to estimate nuclear DNA contents from the exact clone used for this study, a single accession of Mxg named ‘UIUC’, which was originally established as an ornamental variety at the University of Illinois at Urbana-Champaign, but is the source of material used in recent agronomic trials [16]
Given the high abundance of retrotransposons in the genome, these results suggest that small interfering RNA (siRNA) derived from repeats are the major component of the Mxg small-RNA transcriptome
Summary
Miscanthus × giganteus (Mxg) is a perennial grass that produces superior biomass yields in temperate environments. C4 is associated with greater water- and nutrient-use efficiency compared to most plant species [1,2]. These physiological properties contribute to the very high biomass productivity observed from cultivated Andropogoneae crop species, which often exceeds 20 Mg/ha. Another characteristic of the Andropogoneae is a high frequency of polyploidy, with many large and complex genomes. Though typically native to tropical and subtropical climates, Andropogoneae crop species are adapted to a wide diversity of environments and vary in both their life cycle (annual versus perennial) and primary form of harvestable carbon (sugar, grain starch or cellulose)
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