Abstract
In the past the detection and analysis of pathogenic microorganisms in food has required the cultivation, isolation and identification of these organisms. This has been a time consuming endeavor. This approach has also not resolved the problem of those organisms that are viable but non-culturable, that could not be identified by classical culture techniques. With the advent of genome sequencing, more molecular and global strategies for the identification of pathogenic organisms have become available. This presentation will describe and discuss molecular methods based on genomic and proteomic approaches to microbial identification. In addition, tools for analysis of gene expression in a community setting will be presented. Finally, strategies for the discovery of genes expressed during infection and pathogenesis will be considered.
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