Genomic and phenotypic description of the newly isolated human species Collinsella bouchesdurhonensis sp. nov.

Melhem Bilen,Maxime Descartes Mbogning Fonkou,Thi Tien Nguyen,Jérémy Delerce,Ziad Daoud,Pierre‐Edouard Fournier,Mamadou Beye,Nicholas Armstrong,Frédéric Cadoret,Saber Khelaifia,Didier Raoult

doi:10.1002/mbo3.580

Melhem Bilen, Maxime Descartes Mbogning Fonkou + Show 9 more

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https://doi.org/10.1002/mbo3.580

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Abstract

Using culturomics, a recently developed strategy based on diversified culture conditions for the isolation of previously uncultured bacteria, we isolated strain Marseille‐P3296T from a fecal sample of a healthy pygmy female. A multiphasic approach, taxono‐genomics, was used to describe the major characteristics of this anaerobic and gram‐positive bacillus that is unable to sporulate and is not motile. The genome of this bacterium is 1,878,572 bp‐long with a 57.94 mol% G + C content. On the basis of these characteristics and after comparison with its closest phylogenetic neighbors, we are confident that strain Marseille‐P3296T (=CCUG 70328 = CSUR P3296) is the type strain of a novel species for which we propose the name Collinsella bouchesdurhonensis sp. nov.

Highlights

Over the past decade, metagenomics has been extensively adopted to enhance the description of the human gut microbiota (Gill et al, 2006; Ley, Turnbaugh, Klein, & Gordon, 2006; Ley et al, 2005)
We report the isolation of strain Marseille-P3296T from a human stool sample, which we believe to be the representative strain of a new Collinsella species and propose the name Collinsella bouchesdurhonensis (C. bouchesdurhonensis) sp. nov
Strain Marseille-P3296T exhibiting a 16S rRNA divergence greater than 1.35% with its closest phylogenetic neighbor (Kim et al, 2014), we investigated whether it possessed sufficient phenotypic and genomic differences with C. aerofaciens and other Collinsella species to be considered as a new species

Summary

| INTRODUCTION

Metagenomics has been extensively adopted to enhance the description of the human gut microbiota (Gill et al, 2006; Ley, Turnbaugh, Klein, & Gordon, 2006; Ley et al, 2005). Members of the Collinsella genus are gram-positive anaerobic bacilli from the human gut microbiota that were suggested to play a role in health and diseases such as rheumatoid arthritis (Chen et al, 2016) and irritable bowel syndrome (Lee & Bak, 2011). They were isolated from patients with Crohn’s disease, ulcerative colitis, and colon cancer (Whitman et al, 2012).

| MATERIAL AND METHODS

| RESULTS AND DISCUSSION

| CONCLUSION