Abstract
This genus contains both phototrophs and nonphototrophic members. Here, we present a high-quality complete genome of the strain CHu59-6-5T, isolated from a freshwater sediment. The circular chromosome (4.39 Mbp) of the strain CHu59-6-5T has 64.4% G+C content and contains 4240 genes, of which a total of 3918 genes (92.4%) were functionally assigned to the COG (clusters of orthologous groups) database. Functional genes for denitrification (narGHJI, nirK and qnor) were identified on the genomes of the strain CHu59-6-5T, except for N2O reductase (nos) genes for the final step of denitrification. Genes (soxBXAZY) for encoding sulfur oxidation proteins were identified, and the FSD and soxF genes encoding the monomeric flavoproteins which have sulfide dehydrogenase activities were also detected. Lastly, genes for the assembly of two different RND (resistance-nodulation division) type efflux systems and one ABC (ATP-binding cassette) type efflux system were identified in the Rhodoferax sediminis CHu59-6-5T. Phylogenetic analysis based on 16S rRNA sequences and Average Nucleotide Identities (ANI) support the idea that the strain CHu59-6-5T has a close relationship to the genus Rhodoferax. A polyphasic study was done to establish the taxonomic status of the strain CHu59-6-5T. Based on these data, we proposed that the isolate be classified to the genus Rhodoferax as Rhodoferax sediminis sp. nov. with isolate CHu59-6-5T.
Highlights
Rhodoferax species are frequently found in stagnant aquatic environments exposed to light [1], and some have been isolated from fresh water, sewage, sludge and sediments [2,3,4,5,6,7]
The species R. ferrireducens was applied to the area of sustainable energy microbial fuel cells (MFC), where a bacterial suspension was used as a source of electrons for the bacteria [10]
Cell growth was found to occur at temperatures ranging from 4 to 37 ◦C, and quite good growth was observed at 4 ◦C after 10 days; no growth was observed at 0 or 42 ◦C
Summary
Rhodoferax species are frequently found in stagnant aquatic environments exposed to light [1], and some have been isolated from fresh water, sewage, sludge and sediments [2,3,4,5,6,7]. The other two species, R. antarcticus and Rhodoferax saidenbachensis, are psychrotolerant with optimal growth temperatures ranging from 15 to 20 ◦C, but they are capable of growth at temperatures near 0 ◦C [2,4]. Members of the Rhodoferax species are able to grow phototrophically, aerobically and anaerobically. Two species in this genus, R. fermentans and R. antarcticus, grow photoheterotrophically using carbon sources such as acetate, pyruvate, lactate and succinate. A non-phototrophic member in this genus Rhodoferax obtained from freshwater was investigated at the genome and metabolic level, and a polyphasic approach was applied to establish its taxonomic status
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