Genomic and expression characterization of aquaporin genes from Siniperca chuatsi

Abstract

Aquaporins (AQPs) are major intrinsic proteins that form pores in the membranes of biological cells. We first cloned the full-length sequences of aqp0, 1, 3, 4, 7, 8, 9, 10, 11, and 12 genes in Siniperca chuatsi. The 10 S. chuatsi aqp (Sc-aqp) genes included complete open reading frames and exhibited different exon-intron organizations. Sc-aqp1, 3, 8, 9, 10, and 11 were mostly expressed in the gallbladder, gills, gastric cecum, liver, ovaries, and spleen, respectively; Sc-aqp0 and 4 were mostly expressed in larvae at 1 day after hatching and in gastrula; Sc-aqp7 and 12 were mostly expressed in 2K-cell embryos. The expression levels of Sc-aqp1, 3, 7, 8, 9, and 10 after 10 part per thousand (ppt) salt treatment had significantly changed compared with those after 0 ppt salt treatment. Real-time quantitative PCR analysis further showed that in the intestines, the mRNA levels of Sc-aqp1 and 10 significantly decreased by approximately 2.07- and 2.85-fold, respectively, whereas those of Sc-aqp8 and 9 significantly increased by approximately 7.08- and 4.14-fold, respectively. Sc-aqp1, 8, 9, and 10 showed no significant differences in the gills. Sc-aqp3 significantly decreased by approximately 1.51- and 1.67-fold in the gills and intestines, respectively. Sc-aqp7 significantly increased by approximately 4.18- and 7.04-fold in the gills and intestines, respectively. This study was the first to investigate the tissue expression profiles and response to salt stress of aqp genes in S. chuatsi. Moreover, altering diet and suffering from immune stress could cause changes in the expression level of aqps. This study provided valuable reference information for AQPs' roles in osmoregulation in freshwater fish.