Abstract
Using the Genefishing method, we identified seven potential regulatory genes involved in the process of scale morphogenesis in fishes. We further characterized a novel solute carrier protein gene (CcSLC), from the common carp which is differentially expressed in mirror carp and Jianli. The ORF encodes a peptide of 298 amino acids with a molecular mass of 31.5 kDa and a theoretical isoelectric point of 7.49. ScanProsite analysis indicated that it is a putative solute carrier protein that contains a substrate binding site. CcSLC was detected in carp embryos by in situ hybridization in the 70%-epiboly, 6-somite, and 14-somite embryonic stages. Gene expression stopped at the long pec stage. However, CcSLC25a5 was re-expressed during the initiation of scale formation in the regions that were scale covered. These findings provide novel insights into the features of early carp embryo and scale development.
Highlights
Membrane transporters are the gatekeepers for all cells and organelles, controlling uptake and efflux of crucial compounds such as sugars, nucleotides, inorganic ions, and drugs (Hediger et al 2004)
The mirror carp and Jianli have differential gene expression in skin tissues To identify the differentially expressed genes that are associated with development of the skin and appendages, skin samples from mirror carp and Jianli were assayed by Genefishing
Natural variation in scale patterns exists among the common carp species
Summary
Membrane transporters are the gatekeepers for all cells and organelles, controlling uptake and efflux of crucial compounds such as sugars, nucleotides, inorganic ions, and drugs (Hediger et al 2004). They are responsible for substrate movement across both cytoplasmic membranes of cells and internal membranes of organelles (Sreedharan et al 2011). As the largest group of secondary transporters, SLC transporters are becoming the focus of an increasing number of studies because they control transmembrane movement of many types of important substrates. The human genome contains approximately 360 unique SLC protein genes grouped into 48 families (Ren et al 2007; Fredriksson et al 2008). 19 of the SLC gene families have been reported to transport xenobiotics including: organic anion polypeptides (SLCO), oligopeptides (SLC15) (Russel et al 2002; Brandsch et al 2008; Dobson and Kell 2008; Rubio and Daniel 2008), organic anion/cations (SLC22) (Koepsell et al 2007; Ciariboli 2008), and organic cations (SLC47) (Tanihara et al 2007; Moriyama et al 2008; Matsushima et al 2009)
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