Abstract
In MRL/MpJ mice, ovarian mast cells (OMCs) are more abundant than in other mouse strains, and tend to distribute beneath the ovarian surface epithelium at birth. This study investigated the factors regulating the appearance of neonatal OMCs in progeny of the cross between MRL/MpJ and C57BL/6N strains. F1 neonates had less than half the number of OMCs than MRL/MpJ. Interestingly, MRLB6F1 had more neonatal OMCs than B6MRLF1, although they were distributed over comparable areas. Furthermore, in MRL/MpJ fetuses for which parturition was delayed until embryonic day 21.5, the number of OMCs was significantly higher than in age-matched controls at postnatal day 2. These results suggest that the number of OMCs was influenced by the environmental factors during pregnancy. Quantitative trait locus analysis using N2 backcross progeny revealed two significant loci on chromosome 8: D8Mit343–D8Mit312 for the number of OMCs and D8Mit86–D8Mit89 for their distribution, designated as mast cell in the ovary of MRL/MpJ 1 (mcom1) and mcom2, respectively. Among MC migration-associated genes, ovarian expression of chemokine (C-C motif) ligand 17 at mcom1 locus was significantly higher in MRL/MpJ than in C57BL/6N, and positively correlated with the expression of OMC marker genes. These results indicate that the appearance of neonatal OMCs in MRL/MpJ is controlled by environmental factors and filial genetic factors, and that the abundance and distribution of OMCs are regulated by independent filial genetic elements.
Highlights
Mast cells (MCs) reside in most tissues and act as sentinel cells in both innate and adaptive immunity [1]
To determine the pattern of inheritance, the appearance of ovarian mast cells (OMCs) was compared between MRL/MpJ and C57BL/6N mice and their F1 and F2 progeny at postnatal day 0 (P0)
OMC density was 50-fold higher in MRL/MpJ than in C57BL/6N mice (Fig. 1G), while values in F1 and F2 were less than half of that observed in the MRL/MpJ parental strain
Summary
Mast cells (MCs) reside in most tissues and act as sentinel cells in both innate and adaptive immunity [1]. We previously reported other unique characteristics in the reproductive organs of MRL/MpJ mice; i.e., metaphase-specific apoptosis of meiotic spermatocytes, heat shock resistance of spermatocytes found in experimental cryptorchidism, existence of testicular oocytes in newborn males, and development of ovarian cysts originating from the rete ovarii [12,13,14,15]. These phenotypes were closely associated with the genetic background of MRL/MpJ mice, and several susceptibility loci were identified by quantitative trait locus (QTL) analysis [16,17,18,19,20]
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