Abstract
Lactobacillus reuteri (L. reuteri) WHH1689, which was isolated from Chinese traditional highland barley wine, exhibited high survival period at room temperature in drinkable probiotic yogurt. This article aimed to indicate the genes involved in probiotic function of WHH1689 and reveal potential stress resistance based on genomic analysis. Analysis of comparative genome with closely related L. reuteri strains identified special stress adaptation. MUMmer and ACT softwares were applied for collinear analysis, and OrthoMCL program was used for sequence alignment involved in distribution of protein cluster. We identified genes coding for carbohydrate transport and enzymes, carbon metabolism pathway, gastrointestinal tract resistance, adhesive ability, and folic acid biosynthesis, etc. Genome sequence and comparative genome analysis of L. reuteri WHH1689 demonstrated specific genes for genetic adaptation and stress resistance. Tolerance, adhesion, and folate test indicated the strain had multiple probiotics. L. reuteri WHH1689 has the potential to be a probiotic candidate in dairy foods.
Highlights
Probiotics are living microorganisms with adequate amount which have benefit for host health according to FAO/WHO (Manigandan, Mangaiyarkarasi, Hemalatha, Hemalatha, & Murali, 2015)
The circular genome of WHH1689 is comprised of 2,196 genes which were predicted with the average length of 814 bp
A total of 24 genomic islands involved in hundreds of functional genes including transporter, membrane protein, some enzyme, heavy metal resistance, and putative protein gene (Liu et al, 2015; Yoo et al, 2017)
Summary
Probiotics are living microorganisms with adequate amount which have benefit for host health according to FAO/WHO (Manigandan, Mangaiyarkarasi, Hemalatha, Hemalatha, & Murali, 2015). Lactobacillus reuteri is a facultative anaerobic, heterofermentative LAB which is used as autochthonous bacterium in most vertebrates and mammals and is known as improving the allergy constitution and preventing the recurrence of allergy (Shornikova, Casas, Isolauri, Mykkänen, & Vesikari, 1997; Speranza et al, 2018). It has been extensively application for relieving constipation through
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