Abstract
Anopheles sinensis is an important malaria vector in China and other Southeast Asian countries, and the emergence of insecticide resistance in this mosquito poses a serious threat to the efficacy of malaria control programs. The recently published An. sinensis genome and transcriptome provide an opportunity to understand the molecular mechanisms of insecticide resistance. Analysis of the An. sinensis genome revealed 174 detoxification genes, including 93 cytochrome P450s (P450s), 31 glutathione-S-transferases (GSTs), and 50 choline/carboxylesterases (CCEs). The gene number was similar to that in An. gambiae, but represented a decrease of 29% and 42% compared with Aedes aegypti and Culex quinquefasciatus, respectively. The considerable contraction in gene number in Anopheles mosquitoes mainly occurred in two detoxification supergene families, P450s and CCEs. The available An. sinensis transcriptome was also re-analyzed to further identify key resistance-associated detoxification genes. Among 174 detoxification genes, 124 (71%) were detected. Several candidate genes overexpressed in a deltamethrin-resistant strain (DR-strain) were identified as belonging to the CYP4 or CYP6 family of P450s and the Delta GST class. These generated data provide a basis for identifying the resistance-associated genes of An. sinensis at the molecular level.
Highlights
Malaria is a major public health problem in tropical and subtropical regions [1, 2]
A manual review identified the vast majority of these sequences as full-length genes, 24 sequences were identified as partial detoxification genes with high similarities to P450 monooxygenases (P450s) or CCEs
We identified five P450s expressed at lower levels in transcription in the An. sinensis
Summary
Malaria is a major public health problem in tropical and subtropical regions [1, 2]. Malaria is transmitted via the bites of infected female Anopheles mosquitoes, which includes nearly 484 species, distributed in seven subgenera [4, 5]. Anopheles sinensis is one of the major malaria vector mosquitoes in East Asia, ranging from the Philippines to Japan. Increased attention has been paid to this species because of its wide geographic distribution, high density and modest susceptibility to malaria [9, 10].Recently, vivax malaria has re-emerged in the areas where An. sinensis was the main vector in central China and Korea [11, 12].
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