Genomic analysis of chloroplast matK and rbcL gene from Flacourtia inermis Roxb for plant DNA barcoding

K Akilabindu

doi:10.30574/gscbps.2019.9.2.0177

Abstract

Identification of medicinally significant plant species is helpful for their utilization as medicine. Flacourtia inermis Roxb are important medicinal plant. Aim: To Genotypic characterization of Flacourtia inermis through DNA sequence of matK and rbcL gene. Flacourtia inermis Roxb DNA was isolated using NucleoSpin® Plant II Kit (Macherey-Nagel gene extraction) Kit. Isolated DNA was amplified by using PCR (matK and rbcL gene primers). Amplified matK and rbcL gene was sequenced by ABI 3500 DNA analyzer and the sequence quality was checked using sequence scanner software v1. The results exposed basic variability of the sequences. The matK and rcbL gene phylogenic tree was constructed using MEGA 6 software. Flacourtia inermis Roxb DNA was successfully isolated using NucleoSpin® Plant II Kit (Macherey-Nagel gene extraction) Kit and confirmed by agarose gel electrophoresis. Isolated matK and rbcL genomic DNA was successfully amplified by using PCR. From the matK sequence, BLASTn analysis result Flacourtia inermis species showed 99.20% relationship with Flacourtia jangomas and rbcL BLASTn analysis result of Flacourtia inermis species showed100% relationship with Flacourtia jangomas at NCBI database. From this study, the result concluded the matK and rcbL genomes can be used for DNA identification of Flacourtia inermis. The information obtained from the study would be helpful for further research involving genotypic identification of Flacourtia sp.

Highlights

Flacourtia inermis plant belonging to family Flacourtiaceae, is a autotrophic plant found in South India, and found in Malaysia, Indonesia, the Philippines etc
A short genome sequence is used as a molecular marker for identifying the diversity that present among the plants
The gels were visualized in a UV transilluminator (Genei) and the image was captured under UV light using Gel documentation system (Bio-Rad)

Summary

Introduction

Flacourtia inermis plant belonging to family Flacourtiaceae, is a autotrophic plant found in South India, and found in Malaysia, Indonesia, the Philippines etc. [1] The most important task for a botanist is the identification of correct plant species.[2]. Flacourtia inermis plant belonging to family Flacourtiaceae, is a autotrophic plant found in South India, and found in Malaysia, Indonesia, the Philippines etc. [1] The most important task for a botanist is the identification of correct plant species.[2] DNA bar-coding is the standard method for species identification and differentiation. DNA bar-coding technique growing rapidly, and functional tool for species diversity examination and monitoring of the genomic evolution.[3] Flacourtia inermis Roxb is a medium size tree that grow up to 15m height. A short genome sequence is used as a molecular marker for identifying the diversity that present among the plants. Chloroplast and nucleus genes have been used for evaluation sequence differences at genomic level. Chloroplast and nucleus genes have been used for evaluation sequence differences at genomic level. [4]

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