Abstract
We determined the complete genome sequence of Lactobacillus brevis KB290, a probiotic lactic acid bacterium isolated from a traditional Japanese fermented vegetable. The genome contained a 2,395,134-bp chromosome that housed 2,391 protein-coding genes and nine plasmids that together accounted for 191 protein-coding genes. KB290 contained no virulence factor genes, and several genes related to presumptive cell wall-associated polysaccharide biosynthesis and the stress response were present in L. brevis KB290 but not in the closely related L. brevis ATCC 367. Plasmid-curing experiments revealed that the presence of plasmid pKB290-1 was essential for the strain's gastrointestinal tract tolerance and tendency to aggregate. Using next-generation deep sequencing of current and 18-year-old stock strains to detect low frequency variants, we evaluated genome stability. Deep sequencing of four periodic KB290 culture stocks with more than 1,000-fold coverage revealed 3 mutation sites and 37 minority variation sites, indicating long-term stability and providing a useful method for assessing the stability of industrial bacteria at the nucleotide level.
Highlights
Probiotics are live microorganisms that, when administered in adequate amounts, confer a health benefit on the host [1]
Genomic analysis of L. brevis ATCC 367 revealed several genes that likely contribute to its probiotic activity [3,11,12]
We identified 55 copies of transposase genes predominated by the IS30 family, more than found in ATCC 367
Summary
Probiotics are live microorganisms that, when administered in adequate amounts, confer a health benefit on the host [1]. The genomes of many probiotic bacterial strains have been sequenced and analyzed in efforts to reveal the genes or metabolic pathways involved in their health-promoting traits [3,4,5]. Genomic analysis of L. brevis ATCC 367 revealed several genes that likely contribute to its probiotic activity [3,11,12].
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