Abstract
δ-Endotoxin gene of Bacillus thuringiensis HD-1 var kurstaki codes for the insecticidal crystal protein (ICP) specific for lepidopteran insects. Since the N-terminal half of the toxin is sufficient both for insect specificity and toxicity, the coding sequence of this part of the gene Cry IA(b) was amplified by PCR and cloned in pUC19. As there was no expression of immunologically detectable δ-endotoxin in this clone in E. coli , the amplified ICP gene was transferred to an expression vector pGE×2T. Restriction mapping and immunoblotting confirmed the presence and expression of the Cry IA(b) gene. This insert should be suitable for expression in plant system if it is mobilized into a plant binary vector.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
