Abstract
Background: Dipsacus asperoides is a traditional Chinese medicinal crop. The root is generally used as a medicine and is frequently prescribed by Chinese doctors for the treatment of back pain, limb paralysis, flutter trauma, tendon injuries, and fractures. With the rapid development of bioinformatics, research has been focused on this species at the gene or molecular level. For purpose of fleshing out genome information about D. asperoides, in this paper we conducted transcriptome analysis of this species.Principal Findings: To date, many genes encoding enzymes involved in the biosynthesis of triterpenoid saponins in D.asperoides have not been elucidated. Illumina paired-end sequencing was employed to probe D. asperoides's various enzymes associated with the relevant mesostate. A total of 30, 832,805 clean reads and de novo spliced 43,243 unigenes were obtained. Of all unigenes, only 8.27% (3578) were successfully annotated in total of seven public databases: Nr, Nt, Swiss-Prot, GO, KOG, KEGG, and Pfam, which might be attributed to the poor studies on D. asperoides. The candidate genes encoding enzymes involved in triterpenoid saponin biosynthesis were identified and experimentally verified by reverse transcription qPCR, encompassing nine cytochrome P450s and 17 UDP-glucosyltransferases. Specifically, unearthly putative genes involved in the glycosylation of hederagenin were acquired. Simultaneously, 4490 SSRs from 43,243 examined sequences were determined via bioinformatics analysis.Conclusion: This study represents the first report on the use of the Illumina sequence platform on this crop at the transcriptome level. Our findings of candidate genes encoding enzymes involved in Dipsacus saponin VI biosynthes is provide novel information in efforts to further understand the triterpenoid metabolic pathway on this species. The initial genetics resources in this study will contribute significantly to the genetic breeding program of D. asperoides, and are beneficial for clinical diagnosis and treatment.
Highlights
Dipsacus asperoides is a traditional Chinese medicinal crop of the Dipsacaceae family
All of the above contigs were assembled into 43,243 unigenes with a total length of 31,420,741 bp
In the first attempt to reveal biological information on D. asperoides using the Illumina GAII transcriptome technology, we obtained 43,243 unigenes of high quality, as shown by 97.28% Q20 bases, without a reference genome sequence. All these unigenes were successfully annotated in public databases
Summary
Dipsacus asperoides is a traditional Chinese medicinal crop of the Dipsacaceae family. Later generations often use it as a prescription with other compatible medicines It has been used clinically for the treatment of osteoporosis, lassitude in loin and legs, fractures, abortion and dysmenorrheal diseases, as well as Alzheimer’s disease and cancer (Zhang et al, 1997, 2003; Corcelle et al, 2006; Wong et al, 2007; Zhu et al, 2009; Seifert-Klauss and Prior, 2010; Niu et al, 2015). For purpose of fleshing out genome information about D. asperoides, in this paper we conducted transcriptome analysis of this species
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
