Abstract
A Genome-wide analysis of EGR-1 binding sites reveals co-localization with CpG islands and histone H3 lysine 9 binding. SP-1 binding occupancies near EGR-1 binding sites are dramatically altered.
Highlights
Immediate early genes are considered to play important roles in dynamic gene regulatory networks following exposure to appropriate stimuli
Quantitative RT-PCR analysis indicated that Early growth response gene (EGR)-1 mRNA in THP-1 cells was transiently induced by phorbol 12-myristate 13-acetate (PMA) stimulation
Based on the potential EGR1 binding regions derived from the above criteria, we examined the association of the 3,301 early growth response gene 1 (EGR-1) clusters with histone H3 lysine acetylation (H3K9ac) enriched loci and found that more than 75% of EGR-1 binding regions were located within 500 bp of H3K9ac enriched loci (Additional data file 4)
Summary
Immediate early genes are considered to play important roles in dynamic gene regulatory networks following exposure to appropriate stimuli. One of the immediate early genes, early growth response gene 1 (EGR-1), has been implicated in differentiation of human monoblastoma cells along the monocytic commitment following treatment with phorbol ester. Regulatory gene networks, involving specific DNA elements and various transcription regulators, control living cells. To maintain a stable cellular state, multiple cell type-specific transcription regulators interact with DNA binding sites in target genes. The molecular mechanism for cell state changes following exposure to appropriate stimuli has not been fully elucidated, the induction of a set of immediate early genes is thought to constitute the first step in the cellular molecular response to stimulant signals for state changes. The precise function of EGR-1 in monocyte differentiation has not been clearly defined
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
