Abstract
Mitogen-activated protein kinase (MAPK) is a form of serine/threonine protein kinase that activated by extracellular stimulation acting through the MAPK cascade (MAPKKK-MAPKK-MAPK). The MAPK cascade gene family, an important family of protein kinases, plays a vital role in responding to various stresses and hormone signal transduction processes in plants. In this study, we identified 14 CmMAPKs, 6 CmMAPKKs and 64 CmMAPKKKs in melon genome. Based on structural characteristics and a comparison of phylogenetic relationships of MAPK gene families from Arabidopsis, cucumber and watermelon, CmMAPKs and CmMAPKKs were categorized into 4 groups, and CmMAPKKKs were categorized into 3 groups. Furthermore, chromosome location revealed an unevenly distribution on chromosomes of MAPK cascade genes in melon, respectively. Eventually, qRT-PCR analysis showed that all 14 CmMAPKs had different expression patterns under drought, salt, salicylic acid (SA), methyl jasmonate (MeJA), red light (RL), and Podosphaera xanthii (P. xanthii) treatments. Overall, the expression levels of CmMAPK3 and CmMAPK7 under different treatments were higher than those in control. Our study provides an important basis for future functional verification of MAPK genes in regulating responses to stress and signal substance in melon.
Highlights
IntroductionPlants often suffer from various stresses including abiotic stresses (low temperature, drought, salt, etc.) and biotic stresses (insects, weeds, diseases, etc.) which attenuate plant growth, development, and adversely affect the quality and productivity
Plants often suffer from various stresses including abiotic stresses and biotic stresses which attenuate plant growth, development, and adversely affect the quality and productivity
Alternative splices, multiple steps of screening and validation of the conserved domains, we identified 14 CmMAPK genes, 6 CmMAPKK genes, and 64 CmMAPKKK genes
Summary
Plants often suffer from various stresses including abiotic stresses (low temperature, drought, salt, etc.) and biotic stresses (insects, weeds, diseases, etc.) which attenuate plant growth, development, and adversely affect the quality and productivity. It is necessary to consider the damage and response mechanisms of plants under stresses for improving resistance [1].
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