Genome-Wide Identification of Banana Csl Gene Family and Their Different Responses to Low Temperature between Chilling-Sensitive and Tolerant Cultivars.

Weina Yuan,Jian Meng,Zhenting He,Jing Liu,Chunxiang Xu,Houbin Chen,Tong Ning,Ganjun Yi,Tomáš Takáč,Yehuan Tan,Xiaoquan Li

doi:10.3390/plants10010122

Abstract

The cell wall plays an important role in responses to various stresses. The cellulose synthase-like gene (Csl) family has been reported to be involved in the biosynthesis of the hemicellulose backbone. However, little information is available on their involvement in plant tolerance to low-temperature (LT) stress. In this study, a total of 42 Csls were identified in Musa acuminata and clustered into six subfamilies (CslA, CslC, CslD, CslE, CslG, and CslH) according to phylogenetic relationships. The genomic features of MaCsl genes were characterized to identify gene structures, conserved motifs and the distribution among chromosomes. A phylogenetic tree was constructed to show the diversity in these genes. Different changes in hemicellulose content between chilling-tolerant and chilling-sensitive banana cultivars under LT were observed, suggesting that certain types of hemicellulose are involved in LT stress tolerance in banana. Thus, the expression patterns of MaCsl genes in both cultivars after LT treatment were investigated by RNA sequencing (RNA-Seq) technique followed by quantitative real-time PCR (qPCR) validation. The results indicated that MaCslA4/12, MaCslD4 and MaCslE2 are promising candidates determining the chilling tolerance of banana. Our results provide the first genome-wide characterization of the MaCsls in banana, and open the door for further functional studies.

Highlights

IntroductionThe plant cell wall consists of polysaccharides (cellulose, hemicellulose and pectin), proteins and other compounds
The plant cell wall consists of polysaccharides, proteins and other compounds
We conducted a comprehensive analysis of the MaCsl gene family, including the identification of members, phylogenetic relationships, chromosomal distribution, and expression profiles in two low temperatures (LTs)-treated banana cultivars with different tolerances to LT

Summary

Introduction

The plant cell wall consists of polysaccharides (cellulose, hemicellulose and pectin), proteins and other compounds. It plays critical roles in the maintenance of cell integrity, and the regulation of many developmental processes in plants [1,2,3,4,5,6]. Hemicelluloses are a diverse group of complex, non-cellulosic polysaccharides, which constitute approximately one-third of the plant cell wall. The backbones of hemicellulosic polysaccharides in plants are made of the cellulose synthase-like (Csl), which are members of a much larger superfamily of genes referred to as glycosyltransferase 2 [11,12]. Recent research in other flowering plants has identified a new CslM family [15]

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