Genome-Wide Identification, Expression Profile of the TIFY Gene Family in Brassica oleracea var. capitata, and Their Divergent Response to Various Pathogen Infections and Phytohormone Treatments.

Xing Liu,Limei Yang,Cunbao Zhao,Yong Wang,Yangyong Zhang,Zhiyuan Fang,Honghao Lv

doi:10.3390/genes11020127

Abstract

TIFY, a plant-specific gene family with the conserved motif TIF[F/Y]XG, plays important roles in various plant biological processes. Here, a total of 36 TIFY genes were identified in the Brassica oleracea genome and classified into JAZ (22 genes), TIFY (7 genes), ZML (5 genes), and PPD (2 genes) subfamilies based on their conserved motifs, which were distributed unevenly across nine chromosomes with different lengths (339–1077 bp) and exon numbers (1–8). Following phylogenetic analysis with A. thaliana and B. rapa TIFY proteins, ten clades were obtained. The expression of these TIFY genes was organ-specific, with thirteen JAZ genes and two PPD genes showing the highest expression in roots and leaves, respectively. More importantly, the JAZs showed divergent responses to various pathogen infections and different phytohormone treatments. Compared with the susceptible line, most JAZs were activated after Plasmodiophora brassicae infection, while there were both induced and inhibited JAZs after Fusarium oxysporum or Xanthomonas campestris infection in the resistance line, indicating their probably distinct roles in disease resistance or susceptibility. Further, the JAZs were all upregulated after MeJA treatment, but were mostly downregulated after SA/ET treatment. In summary, these results contribute to our understanding of the TIFY gene family, revealing that JAZs may play crucial and divergent roles in phytohormone crosstalk and plant defense.

Highlights

The TIFY family is plant-specific and encodes transcription factors (TFs), which was previously annotated as zinc-finger (C-X2 -C-X20 -C-X2 -C) protein expressing in the inflorescence meristem (ZIM)
36 non-redundant TIFY genes were identified in the D134 genome of B. oleracea, including 22 Jasmonate ZIM (JAZ), 2 PPD, 5 ZML and 7 TIFY proteins
Since phytohormones play a key role in signal transduction when plants encounter pathogens, we investigated the expression of the JAZ genes in cabbage-resistant and cabbage-susceptible lines after pathogen inoculation

Summary

Introduction

The TIFY family is plant-specific and encodes transcription factors (TFs), which was previously annotated as zinc-finger (C-X2 -C-X20 -C-X2 -C) protein expressing in the inflorescence meristem (ZIM)family [1]. The TIFY family is plant-specific and encodes transcription factors (TFs), which was previously annotated as zinc-finger (C-X2 -C-X20 -C-X2 -C) protein expressing in the inflorescence meristem (ZIM). AT4G24470, which encodes a putative TF that contains the CCT domain and the C2C2-GATA zinc finger domain, was the first gene to be characterized as having a ZIM domain [2]. 29 different Arabidopsis loci encoding proteins containing GATA-like zinc fingers were identified by BLAST searches [3]. Genes 2020, 11, 127 databases, and its definition was confusing [5,6] In response to these inconsistencies, Vanholme et al (2007) used ‘TIFY’ instead of ZIM to indicate the most conserved amino acid pattern TIF[F/Y]XG (where X represents any amino acid) [7]

Methods

Results

Discussion

Conclusion