Abstract
Dehydrins (DHNs) play a crucial role in enhancing abiotic stress tolerance in plants. Although DHNs have been identified and characterized in many plants, there is little known about Capsicum annuum L., one of the economically important vegetable crops. In this study, seven CaDHNs in the pepper genome were identified, which could be divided into two classes: YnSKn- and SKn-type, based on their highly conserved domains. Quantitative real-time PCR (qRT-PCR) results showed that the seven DHN genes were expressed in all tissues and might be involved in the growth and development of pepper. The gene expression profiles analysis suggested that most of the CaDHN genes were induced by various stresses (low temperature, salt and mannitol) and signaling molecules (ABA, SA and MeJA). Furthermore, the CaDHN3 (YSK2)-silenced pepper plants showed obvious lower resistance to abiotic stresses (cold, salt and mannitol) than the control plants (TRV2:00). So the CaDHN3 might act as a positive role in resisting abiotic stresses. This study lays the foundation for further studies into the regulation of their expression under various conditions.
Highlights
Unfavorable environmental conditions such as cold, high salinity, or drought, limit the growth, development, and distribution of plants which are unable to move away and reduce agricultural productivity [1]
Pepper DHN genes expressed in all tissues, which suggested that CaDHN genes might be involved in the growth and development of pepper
We found that the CaDHN proteins varied from bp (CaDHN3) gene knockout suppressed the expression of Mn-SOD and POD (Fig 6), which might be lead to more reactive oxygen species (ROS) accumulation in the CaDHN3:silenced pepper than in the TRV2:00 pepper plants under stress conditions
Summary
Unfavorable environmental conditions such as cold, high salinity, or drought, limit the growth, development, and distribution of plants which are unable to move away and reduce agricultural productivity [1]. In response to these stresses, plants employ multiple mechanisms to increase their tolerance to various abiotic stresses, such as accumulation of compatible osmolytes (soluble sugars, glycine betaine,and proline) and synthesis of hydrophilic proteins such as dehydrins (DHNs) [2,3,4]. These proteins, a class of unstructured and high hydrophilic proteins, always contain an abundance of charged and polar amino-acids, such as Gly and Pro, and are free of Cys and Trp
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