Genome-Wide Identification and Expression Analysis of MAPK and MAPKK Gene Family in Pomegranate (Punica Granatum L.)

Yuan Ren,Zhaohe Yuan,Linhui Guo,Dapeng Ge,Jianmei Dong

doi:10.3390/agronomy10071015

Yuan Ren, Zhaohe Yuan + Show 3 more

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https://doi.org/10.3390/agronomy10071015

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Journal: Agronomy	Publication Date: Jul 14, 2020
Citations: 1	License type: CC BY 4.0

Affiliation: Nanjing Forestry University

Abstract

Mitogen-activated protein kinase (MAPK) cascade is involved in the regulation of a series of biological processes in organisms, which are composed of MAPKKKs, MAPKKs, and MAPKs. Although genome-wide analyses of it has been well described in some species, little is known about MAPK and MAPKK genes in pomegranates. In this study, we identified 18 PgMAPKs, 9 PgMAPKKs through a genome-wide search. Chromosome localization showed that 27 genes are distributed on 7 chromosomes with different densities. Multiple sequence alignment and phylogenetic analysis revealed that PgMAPKs and PgMAPKKs could be divided into 4 subfamilies (groups A, B, C, and D), respectively. In addition, exon-introns structural analysis of each candidate gene has indicated high levels of conservation within and between phylogenetic groups. Cis-acting element analysis predicted that PgMAPKs and PgMAPKKs were widely involved in the growth, development, stress and hormone response of pomegranate. Expression profile analyses of PgMAPKs and PgMAPKKs were performed in different tissues (root, leaf, flower and fruit), and PgMAPK13 was significantly expressed in all tissues. To our knowledge, this is the first genome-wide analysis of the MAPK and MAPKK gene family in pomegranate. This study provides valuable information for understanding the classification and functions of pomegranate MAPK signal.

Highlights

In the long process of evolution, plants have evolved complex signal network regulation mechanism to adapt to the biotic and abiotic stress during growth and development [1,2,3,4]
According to the described method, 18 PgMAPKs and 9 PgMAPKKs were obtained from pomegranate whole genome, respectively (Table 2)
Chromosomal location analyses showed that 18 mitogen-activated protein kinase (MAPK) and 9 MAPK kinase (MAPKK) presented on 7 chromosomes (Table 2 and Figure 1)

Summary

Introduction

In the long process of evolution, plants have evolved complex signal network regulation mechanism to adapt to the biotic and abiotic stress during growth and development [1,2,3,4]. The mitogen-activated protein kinase (MAPK) cascade is regarded as one of the typical mechanisms of signal transduction. As broad and relatively conservative evolutionary pathway, it is involved in multiple metabolic pathways such as cell division, development process and defense response in organism [5,6,7]. MAPK cascade is composed of three specific kinases, MAP kinase (MAPK), MAPK kinase (MAPKK), and MAPKK kinase (MAPKKK) [8]. They are activated by phosphorylation and dephosphorylation at specific sites [9]. According to sequence alignment and phylogenetic analysis of model species

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Conclusion