Genome-wide identification and expression analysis of jasmonate ZIM domain gene family in tuber mustard (Brassica juncea var. tumida).

Zhaoming Cai,Jingjing Liao,Diandong Wang,Yuanqing Chen,Maoteng Li

doi:10.1371/journal.pone.0234738

Abstract

Tuber mustard, which is the raw material of Fuling pickle, is a crop with great economic value. However, during growth and development, tuber mustard is frequently attacked by the pathogen Plasmodiophora brassicae and frequently experiences salinity stress. Jasmonic acid (JA) is a hormone related to plant resistance to biotic and abiotic stress. Jasmonate ZIM domain proteins (JAZs) are crucial components of the JA signaling pathway and play important roles in plant responses to biotic and abiotic stress. To date, no information is available about the characteristics of the JAZ family genes in tuber mustard. Here, 38 BjJAZ genes were identified in the whole genome of tuber mustard. The BjJAZ genes are located on 17 of 18 chromosomes in the tuber mustard genome. The gene structures and protein motifs of the BjJAZ genes are conserved between tuber mustard and Arabidopsis. The results of qRT-PCR analysis showed that BjuA030800 was specifically expressed in root, and BjuA007483 was specifically expressed in leaf. In addition, 13 BjJAZ genes were transiently induced by P. brassicae at 12 h, and 7 BjJAZ genes were induced by salt stress from 12 to 24 h. These results provide valuable information for further studies on the role of BjJAZ genes in the regulation of plant growth and development and in the response to biotic and abiotic stress.

Highlights

Tuber mustard is the raw material of Fuling pickle, which has great economic value
The protein sequences in the tuber mustard genome with more than 70% identity to AtJAZ proteins were selected as the candidate homologs of Jasmonate ZIM domain proteins (JAZs), and the JAZ proteins were further identified by gene structure and protein domain conservation analyses
A total of 38 nonredundant BjJAZ genes were identified in the genome of tuber mustard, and the identification was supported by conserved functional domain and multiple sequence alignment analyses

Summary

Introduction

As a Cruciferae plant species, tuber mustard is frequently attacked by soil pathogens, such as Plasmodiophora brassicae. Discovering the mechanism by which tuber mustard responds to pathogens is important for improving the production of this crop. Jasmonic acid (JA) is a plant hormone that is responsible for plant resistance to biotic and abiotic stress. Studying the roles of JA signaling-related genes in the regulation of plant growth and development will shed light on the molecular mechanism underlying the resistance of tuber mustard to pathogens such as P. brassicae. Methyl ester JA (MeJA) and isoleucine-conjugated JA (JA-Ile) are derivatives of JA and have similar functions to JA.

Methods

Results

Conclusion