Abstract
Similar to radical-induced cell death 1 (SROs) is a family of small proteins unique to plants. SRO transcription factors play an important role in plants’ response to biotic and abiotic stresses. In this study, we identified 12 BrSRO genes in Chinese cabbage (Brassica rapa L.). Among them, a comprehensive overview of the SRO gene family is presented, including physical and chemical characteristics, chromosome locations, phylogenetic analysis, gene structures, motif analysis, and cis-element analyses. The number of amino acids of BrSRO genes is between 77–779 aa, isoelectric point changed from 6.02 to 9.6. Of the 12 BrSRO genes, 11 were randomly distributed along the 7 chromosomes, while BrSRO12 was located along unassigned scaffolds. Phylogenetic analysis indicated that the SRO proteins from six species, including Arabidopsis, banana, rice, Solanum lycopersicum, Zea mays, and Chinese cabbage were divided into eleven groups. The exon-rich BrSRO6 and BrSRO12 containing 15 exons were clustered to group K. All 12 genes have motif 2, which indicate that motif 2 is a relatively conservative motif. There are many hormone and stress response elements in BrSRO genes. The relative expression levels of 12 BrSRO genes under high temperature, drought, salt, and low temperature conditions were analyzed by real-time fluorescence quantitative PCR. The results indicated the relative expression level of BrSRO8 was significantly up-regulated when plants were exposed to high temperature. The relative expression levels of BrSRO1, 3, 7, 8, and 9 were higher under low temperature treatment. The up-regulated genes response to drought and salt stresses were BrSRO1, 5, 9 and BrSRO1, 8, respectively. These results indicated that these genes have certain responses to different abiotic stresses. This work has provided a foundation for further functional analyses of SRO genes in Chinese cabbage.
Highlights
When subjected to stresses, plants can survive in complex and diverse environments for stress-induced gene expression
These results suggest that similar to radical-induced cell death proteins (SROs) family may play a crucial role in the growth and development of Chinese cabbage, as well as in various hormones and stress
The family members and functions of SROs are largely unknown in Chinese cabbage
Summary
Plants can survive in complex and diverse environments for stress-induced gene expression. SRO is a family of small proteins unique to plants It plays an important role in plant growth and development and in responding to abiotic stresses, such as salt, drought, and heavy metals. The identification and expression pattern response to various stresses of SRO gene families in Chinese cabbage have not been reported until now. In this study, based on whole genome sequencing results, the members of SRO gene family in Chinese cabbage were identified via a bioinformatics analysis method, and subsequently the physical and chemical properties, evolutionary characteristics of its members, and protein structure were analyzed. Our study provides a foundation for further research on the molecular mechanism of SRO gene mediating physiological growth process and stress response, and a significant basis for the genetic improvement of Chinese cabbage
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